Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell...

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Detalles Bibliográficos
Autores principales: Gao, Yuhua, Li, Xiangchen, Zheng, Dong, Guan, Weijun, Ma, Yuehui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394516/
https://www.ncbi.nlm.nih.gov/pubmed/25782160
http://dx.doi.org/10.3390/ijms16035990
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author Gao, Yuhua
Li, Xiangchen
Zheng, Dong
Guan, Weijun
Ma, Yuehui
author_facet Gao, Yuhua
Li, Xiangchen
Zheng, Dong
Guan, Weijun
Ma, Yuehui
author_sort Gao, Yuhua
collection PubMed
description We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).
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spelling pubmed-43945162015-05-21 Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain Gao, Yuhua Li, Xiangchen Zheng, Dong Guan, Weijun Ma, Yuehui Int J Mol Sci Letter We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). MDPI 2015-03-13 /pmc/articles/PMC4394516/ /pubmed/25782160 http://dx.doi.org/10.3390/ijms16035990 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Letter
Gao, Yuhua
Li, Xiangchen
Zheng, Dong
Guan, Weijun
Ma, Yuehui
Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title_full Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title_fullStr Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title_full_unstemmed Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title_short Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
title_sort isolation of a pluripotent neural stem cell from the embryonic bovine brain
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394516/
https://www.ncbi.nlm.nih.gov/pubmed/25782160
http://dx.doi.org/10.3390/ijms16035990
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AT guanweijun isolationofapluripotentneuralstemcellfromtheembryonicbovinebrain
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