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Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394969/ https://www.ncbi.nlm.nih.gov/pubmed/25633921 http://dx.doi.org/10.3892/mmr.2015.3280 |
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author | WANG, JIN-HANG ZHENG, WEN-WEN CHENG, SHI-TONG LIU, BO-XIN LIU, FU-RONG SONG, JIAN-QING |
author_facet | WANG, JIN-HANG ZHENG, WEN-WEN CHENG, SHI-TONG LIU, BO-XIN LIU, FU-RONG SONG, JIAN-QING |
author_sort | WANG, JIN-HANG |
collection | PubMed |
description | The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15 with monoclonal gammopathy of undetermined significance (MGUS) and 20 normal control (NC) outpatients were selected for the detection of miR-21 and SPRY2 expression using reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was performed to detect the expression of miR-21 and SPRY2 in MM cell lines. The expression of miR-21 in U-266 cells following lipofectamine transfection of fluorescence-labeled miR-21 mimic/inhibitor was observed using a fluorescence microscope and the expression level of SPRY2 in the miR-21 mimic/inhibitor-transfected U-266 cells was detected using western blot analysis. The miR-21 expression level in the circulating serum of the MM patient group was significantly higher (P<0.01) than that of the MGUS and NC groups. The MM cell lines with high endogenous miR-21 expression exhibited an expression level of SPRY2 that was significantly lower than that in the MM cells with low endogenous miR-21 expression. The transfection efficiency of fluorescence-labeled miR-21 mimic/inhibitor was >90%. Compared with the miR-21 expression level in untreated U-266 cells (0.82±0.13), the expression level of miR-21 was increased by 120.2-fold in miR-21 mimic-transfected cells (98.6±14.2; P<0.001) and was decreased by 61.9% in the miR-21 inhibitor-transfected cells (0.37±0.06; P<0.05). The grayscale value of protein bands demonstrated that SPRY2 protein expression significantly decreased in miR-21 mimic-transfected U-266 cells compared with that in the inhibitor-transfected, siRNA-transfected and untreated cells (P<0.01). miR-21 may represent a negative regulator involved in the downregulation of SPRY2 in MM. miR-21 is closely associated with the pathogenesis, progression and prognosis of MM and may thus be used as an indicator of poor MM prognosis. |
format | Online Article Text |
id | pubmed-4394969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-43949692015-04-17 Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma WANG, JIN-HANG ZHENG, WEN-WEN CHENG, SHI-TONG LIU, BO-XIN LIU, FU-RONG SONG, JIAN-QING Mol Med Rep Articles The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15 with monoclonal gammopathy of undetermined significance (MGUS) and 20 normal control (NC) outpatients were selected for the detection of miR-21 and SPRY2 expression using reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was performed to detect the expression of miR-21 and SPRY2 in MM cell lines. The expression of miR-21 in U-266 cells following lipofectamine transfection of fluorescence-labeled miR-21 mimic/inhibitor was observed using a fluorescence microscope and the expression level of SPRY2 in the miR-21 mimic/inhibitor-transfected U-266 cells was detected using western blot analysis. The miR-21 expression level in the circulating serum of the MM patient group was significantly higher (P<0.01) than that of the MGUS and NC groups. The MM cell lines with high endogenous miR-21 expression exhibited an expression level of SPRY2 that was significantly lower than that in the MM cells with low endogenous miR-21 expression. The transfection efficiency of fluorescence-labeled miR-21 mimic/inhibitor was >90%. Compared with the miR-21 expression level in untreated U-266 cells (0.82±0.13), the expression level of miR-21 was increased by 120.2-fold in miR-21 mimic-transfected cells (98.6±14.2; P<0.001) and was decreased by 61.9% in the miR-21 inhibitor-transfected cells (0.37±0.06; P<0.05). The grayscale value of protein bands demonstrated that SPRY2 protein expression significantly decreased in miR-21 mimic-transfected U-266 cells compared with that in the inhibitor-transfected, siRNA-transfected and untreated cells (P<0.01). miR-21 may represent a negative regulator involved in the downregulation of SPRY2 in MM. miR-21 is closely associated with the pathogenesis, progression and prognosis of MM and may thus be used as an indicator of poor MM prognosis. D.A. Spandidos 2015-06 2015-01-29 /pmc/articles/PMC4394969/ /pubmed/25633921 http://dx.doi.org/10.3892/mmr.2015.3280 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WANG, JIN-HANG ZHENG, WEN-WEN CHENG, SHI-TONG LIU, BO-XIN LIU, FU-RONG SONG, JIAN-QING Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title | Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title_full | Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title_fullStr | Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title_full_unstemmed | Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title_short | Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma |
title_sort | correlation between microrna-21 and sprouty homolog 2 gene expression in multiple myeloma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394969/ https://www.ncbi.nlm.nih.gov/pubmed/25633921 http://dx.doi.org/10.3892/mmr.2015.3280 |
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