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Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma

The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15...

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Autores principales: WANG, JIN-HANG, ZHENG, WEN-WEN, CHENG, SHI-TONG, LIU, BO-XIN, LIU, FU-RONG, SONG, JIAN-QING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394969/
https://www.ncbi.nlm.nih.gov/pubmed/25633921
http://dx.doi.org/10.3892/mmr.2015.3280
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author WANG, JIN-HANG
ZHENG, WEN-WEN
CHENG, SHI-TONG
LIU, BO-XIN
LIU, FU-RONG
SONG, JIAN-QING
author_facet WANG, JIN-HANG
ZHENG, WEN-WEN
CHENG, SHI-TONG
LIU, BO-XIN
LIU, FU-RONG
SONG, JIAN-QING
author_sort WANG, JIN-HANG
collection PubMed
description The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15 with monoclonal gammopathy of undetermined significance (MGUS) and 20 normal control (NC) outpatients were selected for the detection of miR-21 and SPRY2 expression using reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was performed to detect the expression of miR-21 and SPRY2 in MM cell lines. The expression of miR-21 in U-266 cells following lipofectamine transfection of fluorescence-labeled miR-21 mimic/inhibitor was observed using a fluorescence microscope and the expression level of SPRY2 in the miR-21 mimic/inhibitor-transfected U-266 cells was detected using western blot analysis. The miR-21 expression level in the circulating serum of the MM patient group was significantly higher (P<0.01) than that of the MGUS and NC groups. The MM cell lines with high endogenous miR-21 expression exhibited an expression level of SPRY2 that was significantly lower than that in the MM cells with low endogenous miR-21 expression. The transfection efficiency of fluorescence-labeled miR-21 mimic/inhibitor was >90%. Compared with the miR-21 expression level in untreated U-266 cells (0.82±0.13), the expression level of miR-21 was increased by 120.2-fold in miR-21 mimic-transfected cells (98.6±14.2; P<0.001) and was decreased by 61.9% in the miR-21 inhibitor-transfected cells (0.37±0.06; P<0.05). The grayscale value of protein bands demonstrated that SPRY2 protein expression significantly decreased in miR-21 mimic-transfected U-266 cells compared with that in the inhibitor-transfected, siRNA-transfected and untreated cells (P<0.01). miR-21 may represent a negative regulator involved in the downregulation of SPRY2 in MM. miR-21 is closely associated with the pathogenesis, progression and prognosis of MM and may thus be used as an indicator of poor MM prognosis.
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spelling pubmed-43949692015-04-17 Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma WANG, JIN-HANG ZHENG, WEN-WEN CHENG, SHI-TONG LIU, BO-XIN LIU, FU-RONG SONG, JIAN-QING Mol Med Rep Articles The aim of the present study was to investigate the expression level of microRNA 21 (miR-21) in the peripheral blood of patients with multiple myeloma (MM) and to investigate the correlation between miR-21 and sprouty homolog 2 (SPRY2) gene expression levels in MM. A total of 30 patients with MM, 15 with monoclonal gammopathy of undetermined significance (MGUS) and 20 normal control (NC) outpatients were selected for the detection of miR-21 and SPRY2 expression using reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was performed to detect the expression of miR-21 and SPRY2 in MM cell lines. The expression of miR-21 in U-266 cells following lipofectamine transfection of fluorescence-labeled miR-21 mimic/inhibitor was observed using a fluorescence microscope and the expression level of SPRY2 in the miR-21 mimic/inhibitor-transfected U-266 cells was detected using western blot analysis. The miR-21 expression level in the circulating serum of the MM patient group was significantly higher (P<0.01) than that of the MGUS and NC groups. The MM cell lines with high endogenous miR-21 expression exhibited an expression level of SPRY2 that was significantly lower than that in the MM cells with low endogenous miR-21 expression. The transfection efficiency of fluorescence-labeled miR-21 mimic/inhibitor was >90%. Compared with the miR-21 expression level in untreated U-266 cells (0.82±0.13), the expression level of miR-21 was increased by 120.2-fold in miR-21 mimic-transfected cells (98.6±14.2; P<0.001) and was decreased by 61.9% in the miR-21 inhibitor-transfected cells (0.37±0.06; P<0.05). The grayscale value of protein bands demonstrated that SPRY2 protein expression significantly decreased in miR-21 mimic-transfected U-266 cells compared with that in the inhibitor-transfected, siRNA-transfected and untreated cells (P<0.01). miR-21 may represent a negative regulator involved in the downregulation of SPRY2 in MM. miR-21 is closely associated with the pathogenesis, progression and prognosis of MM and may thus be used as an indicator of poor MM prognosis. D.A. Spandidos 2015-06 2015-01-29 /pmc/articles/PMC4394969/ /pubmed/25633921 http://dx.doi.org/10.3892/mmr.2015.3280 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, JIN-HANG
ZHENG, WEN-WEN
CHENG, SHI-TONG
LIU, BO-XIN
LIU, FU-RONG
SONG, JIAN-QING
Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title_full Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title_fullStr Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title_full_unstemmed Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title_short Correlation between microRNA-21 and sprouty homolog 2 gene expression in multiple myeloma
title_sort correlation between microrna-21 and sprouty homolog 2 gene expression in multiple myeloma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394969/
https://www.ncbi.nlm.nih.gov/pubmed/25633921
http://dx.doi.org/10.3892/mmr.2015.3280
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