Cargando…
Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes)
Elucidating the phylogenetic relationships of the current but problematic Dasyatidae (Order Myliobatiformes) was the first priority of the current study. Here, we studied three molecular gene markers of 43 species (COI gene), 33 species (ND2 gene) and 34 species (RAG1 gene) of stingrays to draft out...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395009/ https://www.ncbi.nlm.nih.gov/pubmed/25867639 http://dx.doi.org/10.1371/journal.pone.0120518 |
_version_ | 1782366351476654080 |
---|---|
author | Lim, Kean Chong Lim, Phaik-Eem Chong, Ving Ching Loh, Kar-Hoe |
author_facet | Lim, Kean Chong Lim, Phaik-Eem Chong, Ving Ching Loh, Kar-Hoe |
author_sort | Lim, Kean Chong |
collection | PubMed |
description | Elucidating the phylogenetic relationships of the current but problematic Dasyatidae (Order Myliobatiformes) was the first priority of the current study. Here, we studied three molecular gene markers of 43 species (COI gene), 33 species (ND2 gene) and 34 species (RAG1 gene) of stingrays to draft out the phylogenetic tree of the order. Nine character states were identified and used to confirm the molecularly constructed phylogenetic trees. Eight or more clades (at different hierarchical level) were identified for COI, ND2 and RAG1 genes in the Myliobatiformes including four clades containing members of the present Dasyatidae, thus rendering the latter non-monophyletic. The uncorrected p-distance between these four ‘Dasytidae’ clades when compared to the distance between formally known families confirmed that these four clades should be elevated to four separate families. We suggest a revision of the present classification, retaining the Dasyatidae (Dasyatis and Taeniurops species) but adding three new families namely, Neotrygonidae (Neotrygon and Taeniura species), Himanturidae (Himantura species) and Pastinachidae (Pastinachus species). Our result indicated the need to further review the classification of Dasyatis microps. By resolving the non-monophyletic problem, the suite of nine character states enables the natural classification of the Myliobatiformes into at least thirteen families based on morphology. |
format | Online Article Text |
id | pubmed-4395009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43950092015-04-21 Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) Lim, Kean Chong Lim, Phaik-Eem Chong, Ving Ching Loh, Kar-Hoe PLoS One Research Article Elucidating the phylogenetic relationships of the current but problematic Dasyatidae (Order Myliobatiformes) was the first priority of the current study. Here, we studied three molecular gene markers of 43 species (COI gene), 33 species (ND2 gene) and 34 species (RAG1 gene) of stingrays to draft out the phylogenetic tree of the order. Nine character states were identified and used to confirm the molecularly constructed phylogenetic trees. Eight or more clades (at different hierarchical level) were identified for COI, ND2 and RAG1 genes in the Myliobatiformes including four clades containing members of the present Dasyatidae, thus rendering the latter non-monophyletic. The uncorrected p-distance between these four ‘Dasytidae’ clades when compared to the distance between formally known families confirmed that these four clades should be elevated to four separate families. We suggest a revision of the present classification, retaining the Dasyatidae (Dasyatis and Taeniurops species) but adding three new families namely, Neotrygonidae (Neotrygon and Taeniura species), Himanturidae (Himantura species) and Pastinachidae (Pastinachus species). Our result indicated the need to further review the classification of Dasyatis microps. By resolving the non-monophyletic problem, the suite of nine character states enables the natural classification of the Myliobatiformes into at least thirteen families based on morphology. Public Library of Science 2015-04-13 /pmc/articles/PMC4395009/ /pubmed/25867639 http://dx.doi.org/10.1371/journal.pone.0120518 Text en © 2015 Lim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lim, Kean Chong Lim, Phaik-Eem Chong, Ving Ching Loh, Kar-Hoe Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title | Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title_full | Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title_fullStr | Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title_full_unstemmed | Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title_short | Molecular and Morphological Analyses Reveal Phylogenetic Relationships of Stingrays Focusing on the Family Dasyatidae (Myliobatiformes) |
title_sort | molecular and morphological analyses reveal phylogenetic relationships of stingrays focusing on the family dasyatidae (myliobatiformes) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395009/ https://www.ncbi.nlm.nih.gov/pubmed/25867639 http://dx.doi.org/10.1371/journal.pone.0120518 |
work_keys_str_mv | AT limkeanchong molecularandmorphologicalanalysesrevealphylogeneticrelationshipsofstingraysfocusingonthefamilydasyatidaemyliobatiformes AT limphaikeem molecularandmorphologicalanalysesrevealphylogeneticrelationshipsofstingraysfocusingonthefamilydasyatidaemyliobatiformes AT chongvingching molecularandmorphologicalanalysesrevealphylogeneticrelationshipsofstingraysfocusingonthefamilydasyatidaemyliobatiformes AT lohkarhoe molecularandmorphologicalanalysesrevealphylogeneticrelationshipsofstingraysfocusingonthefamilydasyatidaemyliobatiformes |