Cargando…

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bon...

Descripción completa

Detalles Bibliográficos
Autores principales: Sreedhar, Hari, Varma, Vishal K., Nguyen, Peter L., Davidson, Bennett, Akkina, Sanjeev, Guzman, Grace, Setty, Suman, Kajdacsy-Balla, Andre, Walsh, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395079/
https://www.ncbi.nlm.nih.gov/pubmed/25650759
http://dx.doi.org/10.3791/52332
_version_ 1782366364833415168
author Sreedhar, Hari
Varma, Vishal K.
Nguyen, Peter L.
Davidson, Bennett
Akkina, Sanjeev
Guzman, Grace
Setty, Suman
Kajdacsy-Balla, Andre
Walsh, Michael J.
author_facet Sreedhar, Hari
Varma, Vishal K.
Nguyen, Peter L.
Davidson, Bennett
Akkina, Sanjeev
Guzman, Grace
Setty, Suman
Kajdacsy-Balla, Andre
Walsh, Michael J.
author_sort Sreedhar, Hari
collection PubMed
description High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and composition of biomolecules (e.g., lipids, DNA, glycogen, protein, collagen). In an FT-IR image, every pixel within the image comprises an entire Infrared (IR) spectrum that can give information on the biochemical status of the cells that can then be exploited for cell-type or disease-type classification. In this paper, we show: how to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualize FT-IR images. We then present some applications of FT-IR for pathology using the liver and kidney as examples. FT-IR imaging holds exciting applications in providing a novel route to obtain biochemical information from cells and tissue in an entirely label-free non-perturbing route towards giving new insight into biomolecular changes as part of disease processes. Additionally, this biochemical information can potentially allow for objective and automated analysis of certain aspects of disease diagnosis.
format Online
Article
Text
id pubmed-4395079
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MyJove Corporation
record_format MEDLINE/PubMed
spelling pubmed-43950792015-04-24 High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology Sreedhar, Hari Varma, Vishal K. Nguyen, Peter L. Davidson, Bennett Akkina, Sanjeev Guzman, Grace Setty, Suman Kajdacsy-Balla, Andre Walsh, Michael J. J Vis Exp Medicine High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and composition of biomolecules (e.g., lipids, DNA, glycogen, protein, collagen). In an FT-IR image, every pixel within the image comprises an entire Infrared (IR) spectrum that can give information on the biochemical status of the cells that can then be exploited for cell-type or disease-type classification. In this paper, we show: how to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualize FT-IR images. We then present some applications of FT-IR for pathology using the liver and kidney as examples. FT-IR imaging holds exciting applications in providing a novel route to obtain biochemical information from cells and tissue in an entirely label-free non-perturbing route towards giving new insight into biomolecular changes as part of disease processes. Additionally, this biochemical information can potentially allow for objective and automated analysis of certain aspects of disease diagnosis. MyJove Corporation 2015-01-21 /pmc/articles/PMC4395079/ /pubmed/25650759 http://dx.doi.org/10.3791/52332 Text en Copyright © 2015, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Medicine
Sreedhar, Hari
Varma, Vishal K.
Nguyen, Peter L.
Davidson, Bennett
Akkina, Sanjeev
Guzman, Grace
Setty, Suman
Kajdacsy-Balla, Andre
Walsh, Michael J.
High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title_full High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title_fullStr High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title_full_unstemmed High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title_short High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
title_sort high-definition fourier transform infrared (ft-ir) spectroscopic imaging of human tissue sections towards improving pathology
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395079/
https://www.ncbi.nlm.nih.gov/pubmed/25650759
http://dx.doi.org/10.3791/52332
work_keys_str_mv AT sreedharhari highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT varmavishalk highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT nguyenpeterl highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT davidsonbennett highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT akkinasanjeev highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT guzmangrace highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT settysuman highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT kajdacsyballaandre highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology
AT walshmichaelj highdefinitionfouriertransforminfraredftirspectroscopicimagingofhumantissuesectionstowardsimprovingpathology