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Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells

Calcium and phosphoinositide signaling regulate cell division in model systems, but their significance in mammalian cells is unclear. Calcium-binding protein-7 (CaBP7) is a phosphatidylinositol 4-kinaseIIIβ (PI4KIIIβ) inhibitor required during cytokinesis in mammalian cells, hinting at a link betwee...

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Autores principales: Rajamanoharan, Dayani, McCue, Hannah V., Burgoyne, Robert D., Haynes, Lee P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395124/
https://www.ncbi.nlm.nih.gov/pubmed/25717182
http://dx.doi.org/10.1091/mbc.E14-07-1243
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author Rajamanoharan, Dayani
McCue, Hannah V.
Burgoyne, Robert D.
Haynes, Lee P.
author_facet Rajamanoharan, Dayani
McCue, Hannah V.
Burgoyne, Robert D.
Haynes, Lee P.
author_sort Rajamanoharan, Dayani
collection PubMed
description Calcium and phosphoinositide signaling regulate cell division in model systems, but their significance in mammalian cells is unclear. Calcium-binding protein-7 (CaBP7) is a phosphatidylinositol 4-kinaseIIIβ (PI4KIIIβ) inhibitor required during cytokinesis in mammalian cells, hinting at a link between these pathways. Here we characterize a novel association of CaBP7 with lysosomes that cluster at the intercellular bridge during cytokinesis in HeLa cells. We show that CaBP7 regulates lysosome clustering and that PI4KIIIβ is essential for normal cytokinesis. CaBP7 depletion induces lysosome mislocalization, extension of intercellular bridge lifetime, and cytokinesis failure. These data connect phosphoinositide and calcium pathways to lysosome localization and normal cytokinesis in mammalian cells.
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spelling pubmed-43951242015-08-14 Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells Rajamanoharan, Dayani McCue, Hannah V. Burgoyne, Robert D. Haynes, Lee P. Mol Biol Cell Articles Calcium and phosphoinositide signaling regulate cell division in model systems, but their significance in mammalian cells is unclear. Calcium-binding protein-7 (CaBP7) is a phosphatidylinositol 4-kinaseIIIβ (PI4KIIIβ) inhibitor required during cytokinesis in mammalian cells, hinting at a link between these pathways. Here we characterize a novel association of CaBP7 with lysosomes that cluster at the intercellular bridge during cytokinesis in HeLa cells. We show that CaBP7 regulates lysosome clustering and that PI4KIIIβ is essential for normal cytokinesis. CaBP7 depletion induces lysosome mislocalization, extension of intercellular bridge lifetime, and cytokinesis failure. These data connect phosphoinositide and calcium pathways to lysosome localization and normal cytokinesis in mammalian cells. The American Society for Cell Biology 2015-04-15 /pmc/articles/PMC4395124/ /pubmed/25717182 http://dx.doi.org/10.1091/mbc.E14-07-1243 Text en © 2015 Rajamanoharan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Rajamanoharan, Dayani
McCue, Hannah V.
Burgoyne, Robert D.
Haynes, Lee P.
Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title_full Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title_fullStr Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title_full_unstemmed Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title_short Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
title_sort modulation of phosphatidylinositol 4-phosphate levels by cabp7 controls cytokinesis in mammalian cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395124/
https://www.ncbi.nlm.nih.gov/pubmed/25717182
http://dx.doi.org/10.1091/mbc.E14-07-1243
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