Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformat...

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Autores principales: Sharei, Armon, Trifonova, Radiana, Jhunjhunwala, Siddharth, Hartoularos, George C., Eyerman, Alexandra T., Lytton-Jean, Abigail, Angin, Mathieu, Sharma, Siddhartha, Poceviciute, Roberta, Mao, Shirley, Heimann, Megan, Liu, Sophia, Talkar, Tanya, Khan, Omar F., Addo, Marylyn, von Andrian, Ulrich H., Anderson, Daniel G., Langer, Robert, Lieberman, Judy, Jensen, Klavs F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395260/
https://www.ncbi.nlm.nih.gov/pubmed/25875117
http://dx.doi.org/10.1371/journal.pone.0118803
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author Sharei, Armon
Trifonova, Radiana
Jhunjhunwala, Siddharth
Hartoularos, George C.
Eyerman, Alexandra T.
Lytton-Jean, Abigail
Angin, Mathieu
Sharma, Siddhartha
Poceviciute, Roberta
Mao, Shirley
Heimann, Megan
Liu, Sophia
Talkar, Tanya
Khan, Omar F.
Addo, Marylyn
von Andrian, Ulrich H.
Anderson, Daniel G.
Langer, Robert
Lieberman, Judy
Jensen, Klavs F.
author_facet Sharei, Armon
Trifonova, Radiana
Jhunjhunwala, Siddharth
Hartoularos, George C.
Eyerman, Alexandra T.
Lytton-Jean, Abigail
Angin, Mathieu
Sharma, Siddhartha
Poceviciute, Roberta
Mao, Shirley
Heimann, Megan
Liu, Sophia
Talkar, Tanya
Khan, Omar F.
Addo, Marylyn
von Andrian, Ulrich H.
Anderson, Daniel G.
Langer, Robert
Lieberman, Judy
Jensen, Klavs F.
author_sort Sharei, Armon
collection PubMed
description Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
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spelling pubmed-43952602015-04-21 Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells Sharei, Armon Trifonova, Radiana Jhunjhunwala, Siddharth Hartoularos, George C. Eyerman, Alexandra T. Lytton-Jean, Abigail Angin, Mathieu Sharma, Siddhartha Poceviciute, Roberta Mao, Shirley Heimann, Megan Liu, Sophia Talkar, Tanya Khan, Omar F. Addo, Marylyn von Andrian, Ulrich H. Anderson, Daniel G. Langer, Robert Lieberman, Judy Jensen, Klavs F. PLoS One Research Article Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies. Public Library of Science 2015-04-13 /pmc/articles/PMC4395260/ /pubmed/25875117 http://dx.doi.org/10.1371/journal.pone.0118803 Text en © 2015 Sharei et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sharei, Armon
Trifonova, Radiana
Jhunjhunwala, Siddharth
Hartoularos, George C.
Eyerman, Alexandra T.
Lytton-Jean, Abigail
Angin, Mathieu
Sharma, Siddhartha
Poceviciute, Roberta
Mao, Shirley
Heimann, Megan
Liu, Sophia
Talkar, Tanya
Khan, Omar F.
Addo, Marylyn
von Andrian, Ulrich H.
Anderson, Daniel G.
Langer, Robert
Lieberman, Judy
Jensen, Klavs F.
Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title_full Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title_fullStr Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title_full_unstemmed Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title_short Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
title_sort ex vivo cytosolic delivery of functional macromolecules to immune cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395260/
https://www.ncbi.nlm.nih.gov/pubmed/25875117
http://dx.doi.org/10.1371/journal.pone.0118803
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