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Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways

Microtubules (MTs) and associated motors play a central role in nuclear migration, which is crucial for diverse biological functions including cell division, polarity, and sexual reproduction. In this paper, we report a dual mechanism underlying nuclear congression during fission yeast karyogamy upo...

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Autores principales: Scheffler, Kathleen, Minnes, Refael, Fraisier, Vincent, Paoletti, Anne, Tran, Phong T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395489/
https://www.ncbi.nlm.nih.gov/pubmed/25869666
http://dx.doi.org/10.1083/jcb.201409087
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author Scheffler, Kathleen
Minnes, Refael
Fraisier, Vincent
Paoletti, Anne
Tran, Phong T.
author_facet Scheffler, Kathleen
Minnes, Refael
Fraisier, Vincent
Paoletti, Anne
Tran, Phong T.
author_sort Scheffler, Kathleen
collection PubMed
description Microtubules (MTs) and associated motors play a central role in nuclear migration, which is crucial for diverse biological functions including cell division, polarity, and sexual reproduction. In this paper, we report a dual mechanism underlying nuclear congression during fission yeast karyogamy upon mating of haploid cells. Using microfluidic chambers for long-term imaging, we captured the precise timing of nuclear congression and identified two minus end–directed motors operating in parallel in this process. Kinesin-14 Klp2 associated with MTs may cross-link and slide antiparallel MTs emanating from the two nuclei, whereas dynein accumulating at spindle pole bodies (SPBs) may pull MTs nucleated from the opposite SPB. Klp2-dependent nuclear congression proceeds at constant speed, whereas dynein accumulation results in an increase of nuclear velocity over time. Surprisingly, the light intermediate chain Dli1, but not dynactin, is required for this previously unknown function of dynein. We conclude that efficient nuclear congression depends on the cooperation of two minus end–directed motors.
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spelling pubmed-43954892015-10-13 Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways Scheffler, Kathleen Minnes, Refael Fraisier, Vincent Paoletti, Anne Tran, Phong T. J Cell Biol Research Articles Microtubules (MTs) and associated motors play a central role in nuclear migration, which is crucial for diverse biological functions including cell division, polarity, and sexual reproduction. In this paper, we report a dual mechanism underlying nuclear congression during fission yeast karyogamy upon mating of haploid cells. Using microfluidic chambers for long-term imaging, we captured the precise timing of nuclear congression and identified two minus end–directed motors operating in parallel in this process. Kinesin-14 Klp2 associated with MTs may cross-link and slide antiparallel MTs emanating from the two nuclei, whereas dynein accumulating at spindle pole bodies (SPBs) may pull MTs nucleated from the opposite SPB. Klp2-dependent nuclear congression proceeds at constant speed, whereas dynein accumulation results in an increase of nuclear velocity over time. Surprisingly, the light intermediate chain Dli1, but not dynactin, is required for this previously unknown function of dynein. We conclude that efficient nuclear congression depends on the cooperation of two minus end–directed motors. The Rockefeller University Press 2015-04-13 /pmc/articles/PMC4395489/ /pubmed/25869666 http://dx.doi.org/10.1083/jcb.201409087 Text en © 2015 Scheffler et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Scheffler, Kathleen
Minnes, Refael
Fraisier, Vincent
Paoletti, Anne
Tran, Phong T.
Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title_full Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title_fullStr Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title_full_unstemmed Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title_short Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
title_sort microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395489/
https://www.ncbi.nlm.nih.gov/pubmed/25869666
http://dx.doi.org/10.1083/jcb.201409087
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