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RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity

BACKGROUND: Lyme disease is a global public health problem caused by the spirochaete Borrelia burgdorferi. Our previous studies found differences in disease severity between B. burgdorferi B31- and B. garinii SZ-infected mice. We hypothesized that genes that are differentially expressed between Borr...

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Autores principales: Wu, Qiong, Guan, Guiquan, Liu, Zhijie, Li, Youquan, Luo, Jianxun, Yin, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395869/
https://www.ncbi.nlm.nih.gov/pubmed/25886272
http://dx.doi.org/10.1186/s13071-014-0623-2
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author Wu, Qiong
Guan, Guiquan
Liu, Zhijie
Li, Youquan
Luo, Jianxun
Yin, Hong
author_facet Wu, Qiong
Guan, Guiquan
Liu, Zhijie
Li, Youquan
Luo, Jianxun
Yin, Hong
author_sort Wu, Qiong
collection PubMed
description BACKGROUND: Lyme disease is a global public health problem caused by the spirochaete Borrelia burgdorferi. Our previous studies found differences in disease severity between B. burgdorferi B31- and B. garinii SZ-infected mice. We hypothesized that genes that are differentially expressed between Borrelia isolates encode bacterial factors that contribute to disease diversity. METHODS: The present study used high-throughput sequencing technology to characterize and compare the transcriptional profiles of B. burgdorferi B31 and B. garinii SZ cultured in vitro. Real-time quantitative RT-PCR was used to validate selected data from RNA-seq experiments. RESULTS: A total of 731 genes were differentially expressed between B. burgdorferi B31 and B. garinii SZ isolates, including those encoding lipoproteins and purine transport proteins. The fold difference in expression for B. garinii SZ versus B. burgdorferi B31 ranged from 22.07 to 1.01. Expression of the OspA, OspB and DbpB genes were significantly lower in B. garinii SZ compared to B. burgdorferi B31. CONCLUSIONS: The results support the hypothesis that global changes in gene expression underlie differences in Borrelia pathogenicity. The findings also provide an empirical basis for studying the mechanism of action of specific genes as well as their potential usefulness for the diagnosis and management of Lyme disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-014-0623-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-43958692015-04-14 RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity Wu, Qiong Guan, Guiquan Liu, Zhijie Li, Youquan Luo, Jianxun Yin, Hong Parasit Vectors Research BACKGROUND: Lyme disease is a global public health problem caused by the spirochaete Borrelia burgdorferi. Our previous studies found differences in disease severity between B. burgdorferi B31- and B. garinii SZ-infected mice. We hypothesized that genes that are differentially expressed between Borrelia isolates encode bacterial factors that contribute to disease diversity. METHODS: The present study used high-throughput sequencing technology to characterize and compare the transcriptional profiles of B. burgdorferi B31 and B. garinii SZ cultured in vitro. Real-time quantitative RT-PCR was used to validate selected data from RNA-seq experiments. RESULTS: A total of 731 genes were differentially expressed between B. burgdorferi B31 and B. garinii SZ isolates, including those encoding lipoproteins and purine transport proteins. The fold difference in expression for B. garinii SZ versus B. burgdorferi B31 ranged from 22.07 to 1.01. Expression of the OspA, OspB and DbpB genes were significantly lower in B. garinii SZ compared to B. burgdorferi B31. CONCLUSIONS: The results support the hypothesis that global changes in gene expression underlie differences in Borrelia pathogenicity. The findings also provide an empirical basis for studying the mechanism of action of specific genes as well as their potential usefulness for the diagnosis and management of Lyme disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-014-0623-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-13 /pmc/articles/PMC4395869/ /pubmed/25886272 http://dx.doi.org/10.1186/s13071-014-0623-2 Text en © Wu et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Qiong
Guan, Guiquan
Liu, Zhijie
Li, Youquan
Luo, Jianxun
Yin, Hong
RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title_full RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title_fullStr RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title_full_unstemmed RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title_short RNA-Seq-based analysis of changes in Borrelia burgdorferi gene expression linked to pathogenicity
title_sort rna-seq-based analysis of changes in borrelia burgdorferi gene expression linked to pathogenicity
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395869/
https://www.ncbi.nlm.nih.gov/pubmed/25886272
http://dx.doi.org/10.1186/s13071-014-0623-2
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