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Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India

BACKGROUND: Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress o...

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Autores principales: Subramanya, M. D., Pai, Sandeep R., Upadhya, Vinayak, Ankad, Gireesh M., Bhagwat, Shalini S., Hegde, Harsha V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395924/
https://www.ncbi.nlm.nih.gov/pubmed/25878460
http://dx.doi.org/10.4103/0975-9476.146544
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author Subramanya, M. D.
Pai, Sandeep R.
Upadhya, Vinayak
Ankad, Gireesh M.
Bhagwat, Shalini S.
Hegde, Harsha V.
author_facet Subramanya, M. D.
Pai, Sandeep R.
Upadhya, Vinayak
Ankad, Gireesh M.
Bhagwat, Shalini S.
Hegde, Harsha V.
author_sort Subramanya, M. D.
collection PubMed
description BACKGROUND: Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress occurring during treatment of such diseases. OBJECTIVE: The study aimed to evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats, India. MATERIALS AND METHODS: Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia, S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed. RESULTS: Sida cordifolia possessed highest total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g), total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric Reducing Antioxidant Power (FRAP) activity, 2, 2′-Azinobis (3-ethyl Benzo Thiazoline-6-Sulfonic acid) ABTS(+) antioxidant activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC. CONCLUSION: The high contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants.
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spelling pubmed-43959242015-04-15 Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India Subramanya, M. D. Pai, Sandeep R. Upadhya, Vinayak Ankad, Gireesh M. Bhagwat, Shalini S. Hegde, Harsha V. J Ayurveda Integr Med Original Research Article BACKGROUND: Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress occurring during treatment of such diseases. OBJECTIVE: The study aimed to evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats, India. MATERIALS AND METHODS: Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia, S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed. RESULTS: Sida cordifolia possessed highest total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g), total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric Reducing Antioxidant Power (FRAP) activity, 2, 2′-Azinobis (3-ethyl Benzo Thiazoline-6-Sulfonic acid) ABTS(+) antioxidant activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC. CONCLUSION: The high contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4395924/ /pubmed/25878460 http://dx.doi.org/10.4103/0975-9476.146544 Text en Copyright: © Journal of Ayurveda and Integrative Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Subramanya, M. D.
Pai, Sandeep R.
Upadhya, Vinayak
Ankad, Gireesh M.
Bhagwat, Shalini S.
Hegde, Harsha V.
Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title_full Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title_fullStr Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title_full_unstemmed Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title_short Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India
title_sort total polyphenolic contents and in vitro antioxidant properties of eight sida species from western ghats, india
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395924/
https://www.ncbi.nlm.nih.gov/pubmed/25878460
http://dx.doi.org/10.4103/0975-9476.146544
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