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A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

BACKGROUND: Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read DNA sequencing platforms. Although instruments for physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products for e...

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Detalles Bibliográficos
Autores principales:	Shinozuka, Hiroshi, Cogan, Noel O I, Shinozuka, Maiko, Marshall, Alexis, Kay, Pippa, Lin, Yi-Han, Spangenberg, German C, Forster, John W
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396059/ https://www.ncbi.nlm.nih.gov/pubmed/25887558 http://dx.doi.org/10.1186/s12896-015-0139-7

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