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Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats
In prior studies, we described the differential organization of corticostriatal and thalamostriatal inputs to the spines of direct pathway (dSPNs) and indirect pathway striatal projection neurons (iSPNs) of the matrix compartment. In the present electron microscopic (EM) analysis, we have refined un...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396197/ https://www.ncbi.nlm.nih.gov/pubmed/25926776 http://dx.doi.org/10.3389/fnsys.2015.00051 |
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author | Deng, Yunping Lanciego, Jose Goff, Lydia Kerkerian-Le Coulon, Patrice Salin, Pascal Kachidian, Philippe Lei, Wanlong Del Mar, Nobel Reiner, Anton |
author_facet | Deng, Yunping Lanciego, Jose Goff, Lydia Kerkerian-Le Coulon, Patrice Salin, Pascal Kachidian, Philippe Lei, Wanlong Del Mar, Nobel Reiner, Anton |
author_sort | Deng, Yunping |
collection | PubMed |
description | In prior studies, we described the differential organization of corticostriatal and thalamostriatal inputs to the spines of direct pathway (dSPNs) and indirect pathway striatal projection neurons (iSPNs) of the matrix compartment. In the present electron microscopic (EM) analysis, we have refined understanding of the relative amounts of cortical axospinous vs. axodendritic input to the two types of SPNs. Of note, we found that individual dSPNs receive about twice as many axospinous synaptic terminals from IT-type (intratelencephalically projecting) cortical neurons as they do from PT-type (pyramidal tract projecting) cortical neurons. We also found that PT-type axospinous synaptic terminals were about 1.5 times as common on individual iSPNs as IT-type axospinous synaptic terminals. Overall, a higher percentage of IT-type terminals contacted dSPN than iSPN spines, while a higher percentage of PT-type terminals contacted iSPN than dSPN spines. Notably, IT-type axospinous synaptic terminals were significantly larger on iSPN spines than on dSPN spines. By contrast to axospinous input, the axodendritic PT-type input to dSPNs was more substantial than that to iSPNs, and the axodendritic IT-type input appeared to be meager and comparable for both SPN types. The prominent axodendritic PT-type input to dSPNs may accentuate their PT-type responsiveness, and the large size of axospinous IT-type terminals on iSPNs may accentuate their IT-type responsiveness. Using transneuronal labeling with rabies virus to selectively label the cortical neurons with direct input to the dSPNs projecting to the substantia nigra pars reticulata, we found that the input predominantly arose from neurons in the upper layers of motor cortices, in which IT-type perikarya predominate. The differential cortical input to SPNs is likely to play key roles in motor control and motor learning. |
format | Online Article Text |
id | pubmed-4396197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43961972015-04-29 Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats Deng, Yunping Lanciego, Jose Goff, Lydia Kerkerian-Le Coulon, Patrice Salin, Pascal Kachidian, Philippe Lei, Wanlong Del Mar, Nobel Reiner, Anton Front Syst Neurosci Neuroscience In prior studies, we described the differential organization of corticostriatal and thalamostriatal inputs to the spines of direct pathway (dSPNs) and indirect pathway striatal projection neurons (iSPNs) of the matrix compartment. In the present electron microscopic (EM) analysis, we have refined understanding of the relative amounts of cortical axospinous vs. axodendritic input to the two types of SPNs. Of note, we found that individual dSPNs receive about twice as many axospinous synaptic terminals from IT-type (intratelencephalically projecting) cortical neurons as they do from PT-type (pyramidal tract projecting) cortical neurons. We also found that PT-type axospinous synaptic terminals were about 1.5 times as common on individual iSPNs as IT-type axospinous synaptic terminals. Overall, a higher percentage of IT-type terminals contacted dSPN than iSPN spines, while a higher percentage of PT-type terminals contacted iSPN than dSPN spines. Notably, IT-type axospinous synaptic terminals were significantly larger on iSPN spines than on dSPN spines. By contrast to axospinous input, the axodendritic PT-type input to dSPNs was more substantial than that to iSPNs, and the axodendritic IT-type input appeared to be meager and comparable for both SPN types. The prominent axodendritic PT-type input to dSPNs may accentuate their PT-type responsiveness, and the large size of axospinous IT-type terminals on iSPNs may accentuate their IT-type responsiveness. Using transneuronal labeling with rabies virus to selectively label the cortical neurons with direct input to the dSPNs projecting to the substantia nigra pars reticulata, we found that the input predominantly arose from neurons in the upper layers of motor cortices, in which IT-type perikarya predominate. The differential cortical input to SPNs is likely to play key roles in motor control and motor learning. Frontiers Media S.A. 2015-04-14 /pmc/articles/PMC4396197/ /pubmed/25926776 http://dx.doi.org/10.3389/fnsys.2015.00051 Text en Copyright © 2015 Deng, Lanciego, Kerkerian-Le Goff, Coulon, Salin, Kachidian, Lei, Del Mar and Reiner. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Deng, Yunping Lanciego, Jose Goff, Lydia Kerkerian-Le Coulon, Patrice Salin, Pascal Kachidian, Philippe Lei, Wanlong Del Mar, Nobel Reiner, Anton Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title | Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title_full | Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title_fullStr | Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title_full_unstemmed | Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title_short | Differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
title_sort | differential organization of cortical inputs to striatal projection neurons of the matrix compartment in rats |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396197/ https://www.ncbi.nlm.nih.gov/pubmed/25926776 http://dx.doi.org/10.3389/fnsys.2015.00051 |
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