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Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p
In order to determine the potential for allergen to modulate T cell expression of the CysLT(1) receptor and responsiveness to leukotrienes, peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated with D. pteronyssinus allergen (Der p). Baseline CysL...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396553/ https://www.ncbi.nlm.nih.gov/pubmed/25918735 http://dx.doi.org/10.1155/2015/384780 |
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author | Thivierge, Maryse Turcotte, Sylvie Rola-Pleszczynski, Marek Stankova, Jana |
author_facet | Thivierge, Maryse Turcotte, Sylvie Rola-Pleszczynski, Marek Stankova, Jana |
author_sort | Thivierge, Maryse |
collection | PubMed |
description | In order to determine the potential for allergen to modulate T cell expression of the CysLT(1) receptor and responsiveness to leukotrienes, peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated with D. pteronyssinus allergen (Der p). Baseline CysLT(1) expression was similar in both groups of donors, but Der p significantly enhanced CysLT(1) expression in CD4(+) and CD8(+) T cells of only allergic individuals and induced enhanced responsiveness of CD4(+) T cells to LTD(4) in terms of calcium mobilisation. This effect was prevented by the CysLT(1) antagonist MK571. Der p also induced IL-4 and IL-10 production, and neutralizing antibody to IL-4 prevented both the enhanced CysLT(1) expression and the enhanced responsiveness of T cells to LTD(4) induced by Der p. In allergic individuals, Der p also induced T cell proliferation and a Th2-biased phenotype. Our data suggest that, in allergen-sensitized individuals, exposure to allergen can enhance T cell expression of CysLT(1) receptors through a mechanism involving IL-4 production. This, in turn, would induce CD4(+) T cell responsiveness to cysteinyl-leukotrienes and Th2 cell activation. |
format | Online Article Text |
id | pubmed-4396553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43965532015-04-27 Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p Thivierge, Maryse Turcotte, Sylvie Rola-Pleszczynski, Marek Stankova, Jana J Immunol Res Research Article In order to determine the potential for allergen to modulate T cell expression of the CysLT(1) receptor and responsiveness to leukotrienes, peripheral blood mononuclear cells from house dust mite-allergic or nonallergic individuals were incubated with D. pteronyssinus allergen (Der p). Baseline CysLT(1) expression was similar in both groups of donors, but Der p significantly enhanced CysLT(1) expression in CD4(+) and CD8(+) T cells of only allergic individuals and induced enhanced responsiveness of CD4(+) T cells to LTD(4) in terms of calcium mobilisation. This effect was prevented by the CysLT(1) antagonist MK571. Der p also induced IL-4 and IL-10 production, and neutralizing antibody to IL-4 prevented both the enhanced CysLT(1) expression and the enhanced responsiveness of T cells to LTD(4) induced by Der p. In allergic individuals, Der p also induced T cell proliferation and a Th2-biased phenotype. Our data suggest that, in allergen-sensitized individuals, exposure to allergen can enhance T cell expression of CysLT(1) receptors through a mechanism involving IL-4 production. This, in turn, would induce CD4(+) T cell responsiveness to cysteinyl-leukotrienes and Th2 cell activation. Hindawi Publishing Corporation 2015 2015-03-31 /pmc/articles/PMC4396553/ /pubmed/25918735 http://dx.doi.org/10.1155/2015/384780 Text en Copyright © 2015 Maryse Thivierge et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Thivierge, Maryse Turcotte, Sylvie Rola-Pleszczynski, Marek Stankova, Jana Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title | Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title_full | Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title_fullStr | Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title_full_unstemmed | Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title_short | Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p |
title_sort | enhanced cysteinyl-leukotriene type 1 receptor expression in t cells from house dust mite-allergic individuals following stimulation with der p |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396553/ https://www.ncbi.nlm.nih.gov/pubmed/25918735 http://dx.doi.org/10.1155/2015/384780 |
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