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Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients
OBJECTIVES: Helicobacter pylori (H. pylori) infection plays an important role in the carcinogenesis and development of gastric cancer. Eradication of H. pylori can effectively reduce the risk of gastric cancer, but the underlying mechanisms are not fully understood. This study aimed to investigate t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397018/ https://www.ncbi.nlm.nih.gov/pubmed/25875960 http://dx.doi.org/10.1371/journal.pone.0124576 |
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author | Liao, Juan Wen, Shichao Cao, Lipeng Zhou, Yunfeng Feng, Zhisong |
author_facet | Liao, Juan Wen, Shichao Cao, Lipeng Zhou, Yunfeng Feng, Zhisong |
author_sort | Liao, Juan |
collection | PubMed |
description | OBJECTIVES: Helicobacter pylori (H. pylori) infection plays an important role in the carcinogenesis and development of gastric cancer. Eradication of H. pylori can effectively reduce the risk of gastric cancer, but the underlying mechanisms are not fully understood. This study aimed to investigate the effect of eradication of H. pylori on the expression levels of FHIT, IL-8 and P73 in the gastric mucosa of first-degree relatives of gastric cancer patients. METHODS: One hundred and thirty-two patients with functional dyspepsia having first-degree relatives with gastric cancer were prospectively recruited in this study. Nine patients presented with H. pylori infection and family histories of gastric cancer, 61 with H. pylori infection and without family histories of gastric cancer, 6 without H. pylori infection and with family histories of gastric cancer, and 56 without H. pylori infection and family histories of gastric cancer. The protein and mRNA expression levels of FHIT, IL-8 and P73 in gastric mucosa of the subjects were detected by immunohistochemical staining and polymerase chain reaction, respectively. RESULTS: Compared with the patients without H. pylori infection and family histories of gastric cancer, both the protein and mRNA levels of FIHT significantly decreased in patients with H. pylori infection and/or family histories of gastric cancer, and both the protein and mRNA levels of IL-8 significantly increased. After eradication of H. pylori, both the protein and mRNA levels of FHIT were significantly higher, and both the protein and mRNA levels of IL-8 were significantly lower. However, H. pylori infection and family histories of gastric cancer had no major effect on P73 expression. CONCLUSIONS: Down-regulation of FHIT and up-regulation of IL-8 may be involved in the pathogenesis of H. pylori infection in the first-degree relatives of gastric cancer patients. |
format | Online Article Text |
id | pubmed-4397018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43970182015-04-21 Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients Liao, Juan Wen, Shichao Cao, Lipeng Zhou, Yunfeng Feng, Zhisong PLoS One Research Article OBJECTIVES: Helicobacter pylori (H. pylori) infection plays an important role in the carcinogenesis and development of gastric cancer. Eradication of H. pylori can effectively reduce the risk of gastric cancer, but the underlying mechanisms are not fully understood. This study aimed to investigate the effect of eradication of H. pylori on the expression levels of FHIT, IL-8 and P73 in the gastric mucosa of first-degree relatives of gastric cancer patients. METHODS: One hundred and thirty-two patients with functional dyspepsia having first-degree relatives with gastric cancer were prospectively recruited in this study. Nine patients presented with H. pylori infection and family histories of gastric cancer, 61 with H. pylori infection and without family histories of gastric cancer, 6 without H. pylori infection and with family histories of gastric cancer, and 56 without H. pylori infection and family histories of gastric cancer. The protein and mRNA expression levels of FHIT, IL-8 and P73 in gastric mucosa of the subjects were detected by immunohistochemical staining and polymerase chain reaction, respectively. RESULTS: Compared with the patients without H. pylori infection and family histories of gastric cancer, both the protein and mRNA levels of FIHT significantly decreased in patients with H. pylori infection and/or family histories of gastric cancer, and both the protein and mRNA levels of IL-8 significantly increased. After eradication of H. pylori, both the protein and mRNA levels of FHIT were significantly higher, and both the protein and mRNA levels of IL-8 were significantly lower. However, H. pylori infection and family histories of gastric cancer had no major effect on P73 expression. CONCLUSIONS: Down-regulation of FHIT and up-regulation of IL-8 may be involved in the pathogenesis of H. pylori infection in the first-degree relatives of gastric cancer patients. Public Library of Science 2015-04-14 /pmc/articles/PMC4397018/ /pubmed/25875960 http://dx.doi.org/10.1371/journal.pone.0124576 Text en © 2015 Liao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liao, Juan Wen, Shichao Cao, Lipeng Zhou, Yunfeng Feng, Zhisong Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title | Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title_full | Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title_fullStr | Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title_full_unstemmed | Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title_short | Effect of Eradication of Helicobacter pylori on Expression Levels of FHIT, IL-8 and P73 in Gastric Mucosa of First-Degree Relatives of Gastric Cancer Patients |
title_sort | effect of eradication of helicobacter pylori on expression levels of fhit, il-8 and p73 in gastric mucosa of first-degree relatives of gastric cancer patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397018/ https://www.ncbi.nlm.nih.gov/pubmed/25875960 http://dx.doi.org/10.1371/journal.pone.0124576 |
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