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Evaluation of Cell Binding Activities of Leptospira ECM Adhesins

Pathogenic spirochetes of the genus Leptospira are the causative agents of leptospirosis, a zoonotic infection that occurs globally. The bacteria colonize the renal proximal tubules of many animals and are shed in the urine. Contact with the urine, or with water contaminated with the urine of infect...

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Autores principales: Robbins, Gregory T., Hahn, Beth L., Evangelista, Karen V., Padmore, Lavinia, Aranda, Patrick S., Coburn, Jenifer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397020/
https://www.ncbi.nlm.nih.gov/pubmed/25875373
http://dx.doi.org/10.1371/journal.pntd.0003712
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author Robbins, Gregory T.
Hahn, Beth L.
Evangelista, Karen V.
Padmore, Lavinia
Aranda, Patrick S.
Coburn, Jenifer
author_facet Robbins, Gregory T.
Hahn, Beth L.
Evangelista, Karen V.
Padmore, Lavinia
Aranda, Patrick S.
Coburn, Jenifer
author_sort Robbins, Gregory T.
collection PubMed
description Pathogenic spirochetes of the genus Leptospira are the causative agents of leptospirosis, a zoonotic infection that occurs globally. The bacteria colonize the renal proximal tubules of many animals and are shed in the urine. Contact with the urine, or with water contaminated with the urine of infected animals can cause infection of new host animals, including humans. Mechanisms of colonization of the proximal tubule and other tissues are not known, but specific interactions between bacterial adhesins and host substrates are likely to be critical in this process. Several extracellular matrix (ECM) adhesins have been previously identified, but more recently, it has been shown that Leptospira bind more efficiently to cells than ECM. In this work, recombinant forms of five putative Leptospira ECM adhesins, namely LipL32, Loa22, OmpL1, p31/LipL45, and LenA were evaluated for binding to cells as well as an expanded variety of ECM components. Reproducible and significant adhesin activity was demonstrated only for OmpL1, which bound to both mammalian cell lines tested and to glycosaminoglycans (GAGs). While determination of biologically significant bacterial adhesion activity will require generation of site-directed mutant strains, our results suggest that OmpL1 is a strong candidate for future evaluation regarding the roles of the adhesin activity of the protein during L. interrogans infection.
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spelling pubmed-43970202015-04-21 Evaluation of Cell Binding Activities of Leptospira ECM Adhesins Robbins, Gregory T. Hahn, Beth L. Evangelista, Karen V. Padmore, Lavinia Aranda, Patrick S. Coburn, Jenifer PLoS Negl Trop Dis Research Article Pathogenic spirochetes of the genus Leptospira are the causative agents of leptospirosis, a zoonotic infection that occurs globally. The bacteria colonize the renal proximal tubules of many animals and are shed in the urine. Contact with the urine, or with water contaminated with the urine of infected animals can cause infection of new host animals, including humans. Mechanisms of colonization of the proximal tubule and other tissues are not known, but specific interactions between bacterial adhesins and host substrates are likely to be critical in this process. Several extracellular matrix (ECM) adhesins have been previously identified, but more recently, it has been shown that Leptospira bind more efficiently to cells than ECM. In this work, recombinant forms of five putative Leptospira ECM adhesins, namely LipL32, Loa22, OmpL1, p31/LipL45, and LenA were evaluated for binding to cells as well as an expanded variety of ECM components. Reproducible and significant adhesin activity was demonstrated only for OmpL1, which bound to both mammalian cell lines tested and to glycosaminoglycans (GAGs). While determination of biologically significant bacterial adhesion activity will require generation of site-directed mutant strains, our results suggest that OmpL1 is a strong candidate for future evaluation regarding the roles of the adhesin activity of the protein during L. interrogans infection. Public Library of Science 2015-04-14 /pmc/articles/PMC4397020/ /pubmed/25875373 http://dx.doi.org/10.1371/journal.pntd.0003712 Text en © 2015 Robbins et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Robbins, Gregory T.
Hahn, Beth L.
Evangelista, Karen V.
Padmore, Lavinia
Aranda, Patrick S.
Coburn, Jenifer
Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title_full Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title_fullStr Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title_full_unstemmed Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title_short Evaluation of Cell Binding Activities of Leptospira ECM Adhesins
title_sort evaluation of cell binding activities of leptospira ecm adhesins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397020/
https://www.ncbi.nlm.nih.gov/pubmed/25875373
http://dx.doi.org/10.1371/journal.pntd.0003712
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