Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

Epac-based FRET sensors have been widely used for the detection of cAMP concentrations in living cells. Originally developed by us as well as others, we have since then reported several important optimizations that make these sensors favourite among many cell biologists. We here report cloning and c...

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Detalles Bibliográficos
Autores principales: Klarenbeek, Jeffrey, Goedhart, Joachim, van Batenburg, Aernoud, Groenewald, Daniella, Jalink, Kees
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397040/
https://www.ncbi.nlm.nih.gov/pubmed/25875503
http://dx.doi.org/10.1371/journal.pone.0122513

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