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Imaging Fos-Jun Transcription Factor Mobility and Interaction in Live Cells by Single Plane Illumination-Fluorescence Cross Correlation Spectroscopy

We collected mobility and interaction maps of c-Fos-eGFP and c-Jun-mRFP1 transcription factors within living cell nuclei. c-Fos dimerizes with c-Jun to form the transcription activator protein-1 (AP-1) which binds to the specific recognition site. To monitor this process, we used fluorescence cross-...

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Detalles Bibliográficos
Autores principales: Pernuš, Agata, Langowski, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397054/
https://www.ncbi.nlm.nih.gov/pubmed/25875593
http://dx.doi.org/10.1371/journal.pone.0123070