Current Technologies and Related Issues for Mushroom Transformation

Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an a...

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Detalles Bibliográficos
Autores principales: Kim, Sinil, Ha, Byeong-Suk, Ro, Hyeon-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2015
Materias:
Mini-Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397374/
https://www.ncbi.nlm.nih.gov/pubmed/25892908
http://dx.doi.org/10.5941/MYCO.2015.43.1.1
Descripción
Sumario:Mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector DNA, removal of host cell wall, introduction of foreign DNA across the cell membrane, and integration into host genomic DNA or maintenance of an autonomous vector DNA inside the host cell. This review introduces limitations and obstacles related to transformation technologies along with possible solutions. Current methods for cell wall removal and cell membrane permeabilization are summarized together with details of two popular technologies, Agrobacterium tumefaciens-mediated transformation and restriction enzyme-mediated integration.

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