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Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture

PURPOSE: This study aims to investigate the additive effect of the Hedera helix (HH) and Rhizoma coptidis (RC) extracts mixture on antitussive and expectorant activities in animals. MATERIALS AND METHODS: The expectorant assay was performed with phenol red secretion in mice trachea. Mice or guinea p...

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Autores principales: Song, Kee Jae, Shin, Young-June, Lee, Kang Ro, Lee, Eun Jung, Suh, Yun Suk, Kim, Kyung-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Yonsei University College of Medicine 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397455/
https://www.ncbi.nlm.nih.gov/pubmed/25837191
http://dx.doi.org/10.3349/ymj.2015.56.3.819
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author Song, Kee Jae
Shin, Young-June
Lee, Kang Ro
Lee, Eun Jung
Suh, Yun Suk
Kim, Kyung-Su
author_facet Song, Kee Jae
Shin, Young-June
Lee, Kang Ro
Lee, Eun Jung
Suh, Yun Suk
Kim, Kyung-Su
author_sort Song, Kee Jae
collection PubMed
description PURPOSE: This study aims to investigate the additive effect of the Hedera helix (HH) and Rhizoma coptidis (RC) extracts mixture on antitussive and expectorant activities in animals. MATERIALS AND METHODS: The expectorant assay was performed with phenol red secretion in mice trachea. Mice or guinea pigs were randomly divided into groups of 8 each, including negative and positive control groups. After gastric administration of the test extracts in mice, 2.5% phenol red solution (0.2 mL) was intraperitoneally injected. Trachea was dissected and optical density of tracheal secretion was measured. After gastric administration of the test extracts in guinea pigs, the antitussive activities were assessed using a citric acid-induced cough measurement. RESULTS: The extracts of HH and RC significantly increased tracheal secretion and inhibited cough. The mixture of HH and RC extracts in a 1:1 concentration at a dose of 200 mg/kg showed a more potent effect on phenol red secretion (25.25±3.14) and cough inhibition (61.25±5.36) than the individual use of each extracts [phenol red secretion; HH 13.39±4.22 (p=0.000), RC 20.78±2.50 (p=0.010), cough inhibition; HH 9.89±4.14 (p=0.010), RC 30.25±7.69 (p=0.000)]. A 3:1 ratio mixture of HH to RC demonstrated an optimal expectorant effect (p<0.001), and this mixture showed expectorant and antitussive effects in a dose-dependent manner. CONCLUSION: This study provides evidence for antitussive and expectorant effect of a 3:1 mixture of HH and RC, which may be a useful therapeutic option for respiratory diseases.
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spelling pubmed-43974552015-05-01 Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture Song, Kee Jae Shin, Young-June Lee, Kang Ro Lee, Eun Jung Suh, Yun Suk Kim, Kyung-Su Yonsei Med J Original Article PURPOSE: This study aims to investigate the additive effect of the Hedera helix (HH) and Rhizoma coptidis (RC) extracts mixture on antitussive and expectorant activities in animals. MATERIALS AND METHODS: The expectorant assay was performed with phenol red secretion in mice trachea. Mice or guinea pigs were randomly divided into groups of 8 each, including negative and positive control groups. After gastric administration of the test extracts in mice, 2.5% phenol red solution (0.2 mL) was intraperitoneally injected. Trachea was dissected and optical density of tracheal secretion was measured. After gastric administration of the test extracts in guinea pigs, the antitussive activities were assessed using a citric acid-induced cough measurement. RESULTS: The extracts of HH and RC significantly increased tracheal secretion and inhibited cough. The mixture of HH and RC extracts in a 1:1 concentration at a dose of 200 mg/kg showed a more potent effect on phenol red secretion (25.25±3.14) and cough inhibition (61.25±5.36) than the individual use of each extracts [phenol red secretion; HH 13.39±4.22 (p=0.000), RC 20.78±2.50 (p=0.010), cough inhibition; HH 9.89±4.14 (p=0.010), RC 30.25±7.69 (p=0.000)]. A 3:1 ratio mixture of HH to RC demonstrated an optimal expectorant effect (p<0.001), and this mixture showed expectorant and antitussive effects in a dose-dependent manner. CONCLUSION: This study provides evidence for antitussive and expectorant effect of a 3:1 mixture of HH and RC, which may be a useful therapeutic option for respiratory diseases. Yonsei University College of Medicine 2015-05-01 2015-04-01 /pmc/articles/PMC4397455/ /pubmed/25837191 http://dx.doi.org/10.3349/ymj.2015.56.3.819 Text en © Copyright: Yonsei University College of Medicine 2015 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Song, Kee Jae
Shin, Young-June
Lee, Kang Ro
Lee, Eun Jung
Suh, Yun Suk
Kim, Kyung-Su
Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title_full Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title_fullStr Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title_full_unstemmed Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title_short Expectorant and Antitussive Effect of Hedera helix and Rhizoma coptidis Extracts Mixture
title_sort expectorant and antitussive effect of hedera helix and rhizoma coptidis extracts mixture
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397455/
https://www.ncbi.nlm.nih.gov/pubmed/25837191
http://dx.doi.org/10.3349/ymj.2015.56.3.819
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