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Reprogramming with Small Molecules instead of Exogenous Transcription Factors

Induced pluripotent stem cells (iPSCs) could be employed in the creation of patient-specific stem cells, which could subsequently be used in various basic and clinical applications. However, current iPSC methodologies present significant hidden risks with respect to genetic mutations and abnormal ex...

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Detalles Bibliográficos
Autores principales: Lin, Tongxiang, Wu, Shouhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397468/
https://www.ncbi.nlm.nih.gov/pubmed/25922608
http://dx.doi.org/10.1155/2015/794632
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author Lin, Tongxiang
Wu, Shouhai
author_facet Lin, Tongxiang
Wu, Shouhai
author_sort Lin, Tongxiang
collection PubMed
description Induced pluripotent stem cells (iPSCs) could be employed in the creation of patient-specific stem cells, which could subsequently be used in various basic and clinical applications. However, current iPSC methodologies present significant hidden risks with respect to genetic mutations and abnormal expression which are a barrier in realizing the full potential of iPSCs. A chemical approach is thought to be a promising strategy for safety and efficiency of iPSC generation. Many small molecules have been identified that can be used in place of exogenous transcription factors and significantly improve iPSC reprogramming efficiency and quality. Recent studies have shown that the use of small molecules results in the generation of chemically induced pluripotent stem cells from mouse embryonic fibroblast cells. These studies might lead to new areas of stem cell research and medical applications, not only human iPSC by chemicals alone, but also safe generation of somatic stem cells for cell based clinical trials and other researches. In this paper, we have reviewed the recent advances in small molecule approaches for the generation of iPSCs.
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spelling pubmed-43974682015-04-28 Reprogramming with Small Molecules instead of Exogenous Transcription Factors Lin, Tongxiang Wu, Shouhai Stem Cells Int Review Article Induced pluripotent stem cells (iPSCs) could be employed in the creation of patient-specific stem cells, which could subsequently be used in various basic and clinical applications. However, current iPSC methodologies present significant hidden risks with respect to genetic mutations and abnormal expression which are a barrier in realizing the full potential of iPSCs. A chemical approach is thought to be a promising strategy for safety and efficiency of iPSC generation. Many small molecules have been identified that can be used in place of exogenous transcription factors and significantly improve iPSC reprogramming efficiency and quality. Recent studies have shown that the use of small molecules results in the generation of chemically induced pluripotent stem cells from mouse embryonic fibroblast cells. These studies might lead to new areas of stem cell research and medical applications, not only human iPSC by chemicals alone, but also safe generation of somatic stem cells for cell based clinical trials and other researches. In this paper, we have reviewed the recent advances in small molecule approaches for the generation of iPSCs. Hindawi Publishing Corporation 2015 2015-04-01 /pmc/articles/PMC4397468/ /pubmed/25922608 http://dx.doi.org/10.1155/2015/794632 Text en Copyright © 2015 T. Lin and S. Wu. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Lin, Tongxiang
Wu, Shouhai
Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title_full Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title_fullStr Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title_full_unstemmed Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title_short Reprogramming with Small Molecules instead of Exogenous Transcription Factors
title_sort reprogramming with small molecules instead of exogenous transcription factors
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397468/
https://www.ncbi.nlm.nih.gov/pubmed/25922608
http://dx.doi.org/10.1155/2015/794632
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