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Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media
INTRODUCTION: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397546/ https://www.ncbi.nlm.nih.gov/pubmed/25878411 http://dx.doi.org/10.4103/0970-1591.152809 |
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author | Nath, Nilima Saraswat, Sumit K. Jain, Shashank Koteshwar, Sridhar |
author_facet | Nath, Nilima Saraswat, Sumit K. Jain, Shashank Koteshwar, Sridhar |
author_sort | Nath, Nilima |
collection | PubMed |
description | INTRODUCTION: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-engineered grafts. The stricture wound healing process after urethroplasty is known to be mediated by an interaction between keratinocyte and fibroblasts; however, the underlying mechanisms are not studied in detail yet. We investigated the influence of epithelial cell-conditioned medium (ECCM) (obtained from confluent penile skin, buccal mucosa and urethral cell cultures) on the proliferation and migration of stricture fibroblasts using an in vitro scratch assay. MATERIALS AND METHODS: ECCM was collected from confluent primary epithelial cell cultures of three different human biopsies (penile skin, buccal mucosa and urethral mucosa), whereas stricture fibroblasts were isolated from human urethral stricture biopsies. The effect of ECCM on stricture fibroblasts’ proliferation and migration into the scratch was observed using a standard in vitro scratch assay over a period of 3 days. Four experiments were performed independently using four stricture fibroblasts from four patients and ECCM was collected from 12 different patients’ primary cell cultures. RESULTS: ECCM from primary epithelial cells cultures obtained from penile skin, buccal mucosa and urethra inhibited stricture fibroblasts’ proliferation and migration in the in vitro scratch assay. CONCLUSION: These results demonstrate the ability of ECCM to inhibit the proliferation and migration of stricture fibroblasts and present it as an effective adjunct in urethroplasty, which may influence stricture wound healing and inhibit the recurrence of stricture. |
format | Online Article Text |
id | pubmed-4397546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43975462015-04-15 Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media Nath, Nilima Saraswat, Sumit K. Jain, Shashank Koteshwar, Sridhar Indian J Urol Original Article INTRODUCTION: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-engineered grafts. The stricture wound healing process after urethroplasty is known to be mediated by an interaction between keratinocyte and fibroblasts; however, the underlying mechanisms are not studied in detail yet. We investigated the influence of epithelial cell-conditioned medium (ECCM) (obtained from confluent penile skin, buccal mucosa and urethral cell cultures) on the proliferation and migration of stricture fibroblasts using an in vitro scratch assay. MATERIALS AND METHODS: ECCM was collected from confluent primary epithelial cell cultures of three different human biopsies (penile skin, buccal mucosa and urethral mucosa), whereas stricture fibroblasts were isolated from human urethral stricture biopsies. The effect of ECCM on stricture fibroblasts’ proliferation and migration into the scratch was observed using a standard in vitro scratch assay over a period of 3 days. Four experiments were performed independently using four stricture fibroblasts from four patients and ECCM was collected from 12 different patients’ primary cell cultures. RESULTS: ECCM from primary epithelial cells cultures obtained from penile skin, buccal mucosa and urethra inhibited stricture fibroblasts’ proliferation and migration in the in vitro scratch assay. CONCLUSION: These results demonstrate the ability of ECCM to inhibit the proliferation and migration of stricture fibroblasts and present it as an effective adjunct in urethroplasty, which may influence stricture wound healing and inhibit the recurrence of stricture. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4397546/ /pubmed/25878411 http://dx.doi.org/10.4103/0970-1591.152809 Text en Copyright: © Indian Journal of Urology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nath, Nilima Saraswat, Sumit K. Jain, Shashank Koteshwar, Sridhar Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title_full | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title_fullStr | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title_full_unstemmed | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title_short | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
title_sort | inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397546/ https://www.ncbi.nlm.nih.gov/pubmed/25878411 http://dx.doi.org/10.4103/0970-1591.152809 |
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