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3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma

PURPOSE: Although the lamina cribrosa (LC) is the primary site of axonal damage in glaucoma, adequate methods to image and measure it are currently lacking. Here, we describe a noninvasive, in vivo method of evaluating the LC, based on swept-source optical coherence tomography (SS-OCT), and determin...

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Autores principales: Omodaka, Kazuko, Horii, Takaaki, Takahashi, Seri, Kikawa, Tsutomu, Matsumoto, Akiko, Shiga, Yukihiro, Maruyama, Kazuichi, Yuasa, Tetsuya, Akiba, Masahiro, Nakazawa, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398555/
https://www.ncbi.nlm.nih.gov/pubmed/25875096
http://dx.doi.org/10.1371/journal.pone.0122347
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author Omodaka, Kazuko
Horii, Takaaki
Takahashi, Seri
Kikawa, Tsutomu
Matsumoto, Akiko
Shiga, Yukihiro
Maruyama, Kazuichi
Yuasa, Tetsuya
Akiba, Masahiro
Nakazawa, Toru
author_facet Omodaka, Kazuko
Horii, Takaaki
Takahashi, Seri
Kikawa, Tsutomu
Matsumoto, Akiko
Shiga, Yukihiro
Maruyama, Kazuichi
Yuasa, Tetsuya
Akiba, Masahiro
Nakazawa, Toru
author_sort Omodaka, Kazuko
collection PubMed
description PURPOSE: Although the lamina cribrosa (LC) is the primary site of axonal damage in glaucoma, adequate methods to image and measure it are currently lacking. Here, we describe a noninvasive, in vivo method of evaluating the LC, based on swept-source optical coherence tomography (SS-OCT), and determine this method’s ability to quantify LC thickness. METHODS: This study comprised 54 eyes, including normal (n = 18), preperimetric glaucoma (PPG; n = 18), and normal tension glaucoma (NTG; n = 18) eyes. We used SS-OCT to obtain 3 x 3 mm cube scans of an area centered on the optic disc, and then synchronized reconstructed B- and en-face images from this data. We identified the LC in these B-scan images by marking the visible borders of the LC pores. We marked points on the anterior and posterior borders of the LC in 12 B-scan images in order to create a skeleton model of the LC. Finally, we used B-spline interpolation to form a 3D model of the LC, including only reliably measured scan areas. We calculated the average LC thickness (avgLCT) in this model and used Spearman's rank correlation coefficient to compare it with circumpapillary retinal nerve fiber layer thickness (cpRNFLT). RESULTS: We found that the correlation coefficient of avgLCT and cpRNFLT was 0.64 (p < 0.01). The coefficient of variation for avgLCT was 5.1%. AvgLCT differed significantly in the groups (normal: 282.6 ± 20.6 μm, PPG: 261.4 ± 15.8 μm, NTG: 232.6 ± 33.3 μm). The normal, PPG and NTG groups did not significantly differ in age, sex, refractive error or intraocular pressure (IOP), although the normal and NTG groups differed significantly in cpRNFLT and Humphrey field analyzer measurements of mean deviation. CONCLUSION: Thus, our results indicate that the parameters of our newly developed method of measuring LC thickness with SS-OCT may provide useful and important data for glaucoma diagnosis and research.
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spelling pubmed-43985552015-04-21 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma Omodaka, Kazuko Horii, Takaaki Takahashi, Seri Kikawa, Tsutomu Matsumoto, Akiko Shiga, Yukihiro Maruyama, Kazuichi Yuasa, Tetsuya Akiba, Masahiro Nakazawa, Toru PLoS One Research Article PURPOSE: Although the lamina cribrosa (LC) is the primary site of axonal damage in glaucoma, adequate methods to image and measure it are currently lacking. Here, we describe a noninvasive, in vivo method of evaluating the LC, based on swept-source optical coherence tomography (SS-OCT), and determine this method’s ability to quantify LC thickness. METHODS: This study comprised 54 eyes, including normal (n = 18), preperimetric glaucoma (PPG; n = 18), and normal tension glaucoma (NTG; n = 18) eyes. We used SS-OCT to obtain 3 x 3 mm cube scans of an area centered on the optic disc, and then synchronized reconstructed B- and en-face images from this data. We identified the LC in these B-scan images by marking the visible borders of the LC pores. We marked points on the anterior and posterior borders of the LC in 12 B-scan images in order to create a skeleton model of the LC. Finally, we used B-spline interpolation to form a 3D model of the LC, including only reliably measured scan areas. We calculated the average LC thickness (avgLCT) in this model and used Spearman's rank correlation coefficient to compare it with circumpapillary retinal nerve fiber layer thickness (cpRNFLT). RESULTS: We found that the correlation coefficient of avgLCT and cpRNFLT was 0.64 (p < 0.01). The coefficient of variation for avgLCT was 5.1%. AvgLCT differed significantly in the groups (normal: 282.6 ± 20.6 μm, PPG: 261.4 ± 15.8 μm, NTG: 232.6 ± 33.3 μm). The normal, PPG and NTG groups did not significantly differ in age, sex, refractive error or intraocular pressure (IOP), although the normal and NTG groups differed significantly in cpRNFLT and Humphrey field analyzer measurements of mean deviation. CONCLUSION: Thus, our results indicate that the parameters of our newly developed method of measuring LC thickness with SS-OCT may provide useful and important data for glaucoma diagnosis and research. Public Library of Science 2015-04-15 /pmc/articles/PMC4398555/ /pubmed/25875096 http://dx.doi.org/10.1371/journal.pone.0122347 Text en © 2015 Omodaka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Omodaka, Kazuko
Horii, Takaaki
Takahashi, Seri
Kikawa, Tsutomu
Matsumoto, Akiko
Shiga, Yukihiro
Maruyama, Kazuichi
Yuasa, Tetsuya
Akiba, Masahiro
Nakazawa, Toru
3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title_full 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title_fullStr 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title_full_unstemmed 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title_short 3D Evaluation of the Lamina Cribrosa with Swept-Source Optical Coherence Tomography in Normal Tension Glaucoma
title_sort 3d evaluation of the lamina cribrosa with swept-source optical coherence tomography in normal tension glaucoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398555/
https://www.ncbi.nlm.nih.gov/pubmed/25875096
http://dx.doi.org/10.1371/journal.pone.0122347
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