Imaging and force measurement of LDL and HDL by AFM in air and liquid

The size and biomechanical properties of lipoproteins are tightly correlated with their structures/functions. While atomic force microscopy (AFM) has been used to image lipoproteins the force measurement of these nano-sized particles is missing. We detected that the sizes of LDL and HDL in liquid ar...

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Detalles Bibliográficos
Autores principales: Gan, Chaoye, Ao, Meiying, Liu, Zhanghua, Chen, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398754/
https://www.ncbi.nlm.nih.gov/pubmed/25893163
http://dx.doi.org/10.1016/j.fob.2015.03.014
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author Gan, Chaoye
Ao, Meiying
Liu, Zhanghua
Chen, Yong
author_facet Gan, Chaoye
Ao, Meiying
Liu, Zhanghua
Chen, Yong
author_sort Gan, Chaoye
collection PubMed
description The size and biomechanical properties of lipoproteins are tightly correlated with their structures/functions. While atomic force microscopy (AFM) has been used to image lipoproteins the force measurement of these nano-sized particles is missing. We detected that the sizes of LDL and HDL in liquid are close to the commonly known values. The Young’s modulus of LDL or HDL is ∼0.4 GPa which is similar to that of some viral capsids or nanovesicles but greatly larger than that of various liposomes. The adhesive force of LDL or HDL is small (∼200 pN). The comparison of AFM detection in air and liquid was also performed which is currently lacking. Our data may provide useful information for better understanding and AFM detection of lipoproteins.
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spelling pubmed-43987542015-04-17 Imaging and force measurement of LDL and HDL by AFM in air and liquid Gan, Chaoye Ao, Meiying Liu, Zhanghua Chen, Yong FEBS Open Bio Article The size and biomechanical properties of lipoproteins are tightly correlated with their structures/functions. While atomic force microscopy (AFM) has been used to image lipoproteins the force measurement of these nano-sized particles is missing. We detected that the sizes of LDL and HDL in liquid are close to the commonly known values. The Young’s modulus of LDL or HDL is ∼0.4 GPa which is similar to that of some viral capsids or nanovesicles but greatly larger than that of various liposomes. The adhesive force of LDL or HDL is small (∼200 pN). The comparison of AFM detection in air and liquid was also performed which is currently lacking. Our data may provide useful information for better understanding and AFM detection of lipoproteins. Elsevier 2015-04-07 /pmc/articles/PMC4398754/ /pubmed/25893163 http://dx.doi.org/10.1016/j.fob.2015.03.014 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Gan, Chaoye
Ao, Meiying
Liu, Zhanghua
Chen, Yong
Imaging and force measurement of LDL and HDL by AFM in air and liquid
title Imaging and force measurement of LDL and HDL by AFM in air and liquid
title_full Imaging and force measurement of LDL and HDL by AFM in air and liquid
title_fullStr Imaging and force measurement of LDL and HDL by AFM in air and liquid
title_full_unstemmed Imaging and force measurement of LDL and HDL by AFM in air and liquid
title_short Imaging and force measurement of LDL and HDL by AFM in air and liquid
title_sort imaging and force measurement of ldl and hdl by afm in air and liquid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4398754/
https://www.ncbi.nlm.nih.gov/pubmed/25893163
http://dx.doi.org/10.1016/j.fob.2015.03.014
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