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Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis

Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoi...

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Autores principales: Fazlollahi, Farbod, Kongmanas, Kessiri, Tanphaichitr, Nongnuj, Suh, Jeffrey, Faull, Kym, Gopen, Quinton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Publicações Ltda 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399193/
https://www.ncbi.nlm.nih.gov/pubmed/25992170
http://dx.doi.org/10.1055/s-0034-1396522
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author Fazlollahi, Farbod
Kongmanas, Kessiri
Tanphaichitr, Nongnuj
Suh, Jeffrey
Faull, Kym
Gopen, Quinton
author_facet Fazlollahi, Farbod
Kongmanas, Kessiri
Tanphaichitr, Nongnuj
Suh, Jeffrey
Faull, Kym
Gopen, Quinton
author_sort Fazlollahi, Farbod
collection PubMed
description Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoiditis. Methods The bone dust created during mastoidectomy, as well as the mastoid tissue, was analyzed from seven patients. Bone dust was also collected and analyzed in an additional four otologic cases (parotidectomy requiring mastoidectomy). Samples were subjected to a modified Bligh/Dyer lipid extraction, then high-performance thin-layer chromatography (HPTLC), combined gas chromatography/electron impact-mass spectrometry (GC/EI-MS), and flow-injection/electrospray ionization-tandem mass spectrometry (FI/ESI-MSMS). Data were analyzed for identification and profiling of major lipid components. Results HPTLC revealed the presence of various lipid classes, including phosphatidylcholines, cholesterol, and triacylglycerols. GC/EI-MS analysis revealed the presence of cholesterol and several fatty acids. FI/ESI-MSMS analysis revealed a host of phosphatidylcholines, phosphatidylethanolamines, and cholesteryl esters. Conclusion We used a lipidomics approach to develop an efficient (both in time and tissue amount) methodology for analysis of these tissues, identify the most abundant and common lipid species, and create a base of knowledge from which more focused endeavors in biomarker discovery can emerge. In an effort toward improved patient categorization and individualized intervention, the ultimate goal of this work is to correlate these lipid molecules to disease state and progression. This is the first reported study of its kind on these tissues.
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spelling pubmed-43991932015-05-19 Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis Fazlollahi, Farbod Kongmanas, Kessiri Tanphaichitr, Nongnuj Suh, Jeffrey Faull, Kym Gopen, Quinton Int Arch Otorhinolaryngol Article Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoiditis. Methods The bone dust created during mastoidectomy, as well as the mastoid tissue, was analyzed from seven patients. Bone dust was also collected and analyzed in an additional four otologic cases (parotidectomy requiring mastoidectomy). Samples were subjected to a modified Bligh/Dyer lipid extraction, then high-performance thin-layer chromatography (HPTLC), combined gas chromatography/electron impact-mass spectrometry (GC/EI-MS), and flow-injection/electrospray ionization-tandem mass spectrometry (FI/ESI-MSMS). Data were analyzed for identification and profiling of major lipid components. Results HPTLC revealed the presence of various lipid classes, including phosphatidylcholines, cholesterol, and triacylglycerols. GC/EI-MS analysis revealed the presence of cholesterol and several fatty acids. FI/ESI-MSMS analysis revealed a host of phosphatidylcholines, phosphatidylethanolamines, and cholesteryl esters. Conclusion We used a lipidomics approach to develop an efficient (both in time and tissue amount) methodology for analysis of these tissues, identify the most abundant and common lipid species, and create a base of knowledge from which more focused endeavors in biomarker discovery can emerge. In an effort toward improved patient categorization and individualized intervention, the ultimate goal of this work is to correlate these lipid molecules to disease state and progression. This is the first reported study of its kind on these tissues. Thieme Publicações Ltda 2014-12-08 2015-04 /pmc/articles/PMC4399193/ /pubmed/25992170 http://dx.doi.org/10.1055/s-0034-1396522 Text en © Thieme Medical Publishers
spellingShingle Article
Fazlollahi, Farbod
Kongmanas, Kessiri
Tanphaichitr, Nongnuj
Suh, Jeffrey
Faull, Kym
Gopen, Quinton
Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title_full Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title_fullStr Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title_full_unstemmed Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title_short Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
title_sort lipidomic profiling of mastoid bone and tissue from patients with chronic otomastoiditis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399193/
https://www.ncbi.nlm.nih.gov/pubmed/25992170
http://dx.doi.org/10.1055/s-0034-1396522
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