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Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech

Unlike mammals, the CNS of the medicinal leech can regenerate damaged neurites, thus restoring neural functions after lesion. We previously demonstrated that the injured leech nerve cord is able to mount an immune response promoting the regenerative processes. Indeed neurons and microglia express se...

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Autores principales: Rodet, F., Tasiemski, A., Boidin-Wichlacz, C., Van Camp, C., Vuillaume, C., Slomianny, C., Salzet, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399414/
https://www.ncbi.nlm.nih.gov/pubmed/25880897
http://dx.doi.org/10.1038/srep09624
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author Rodet, F.
Tasiemski, A.
Boidin-Wichlacz, C.
Van Camp, C.
Vuillaume, C.
Slomianny, C.
Salzet, M.
author_facet Rodet, F.
Tasiemski, A.
Boidin-Wichlacz, C.
Van Camp, C.
Vuillaume, C.
Slomianny, C.
Salzet, M.
author_sort Rodet, F.
collection PubMed
description Unlike mammals, the CNS of the medicinal leech can regenerate damaged neurites, thus restoring neural functions after lesion. We previously demonstrated that the injured leech nerve cord is able to mount an immune response promoting the regenerative processes. Indeed neurons and microglia express sensing receptors like Hm-TLR1, a leech TLR ortholog, associated with chemokine release in response to a septic challenge or lesion. To gain insights into the TLR signaling pathways involved during these neuroimmune responses, members of the MyD88 family were investigated. In the present study, we report the characterization of Hm-MyD88 and Hm-SARM. The expression of their encoding gene was strongly regulated in leech CNS not only upon immune challenge but also during CNS repair, suggesting their involvement in both processes. This work also showed for the first time that differentiated neurons of the CNS could respond to LPS through a MyD88-dependent signalling pathway, while in mammals, studies describing the direct effect of LPS on neurons and the outcomes of such treatment are scarce and controversial. In the present study, we established that this PAMP induced the relocalization of Hm-MyD88 in isolated neurons.
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spelling pubmed-43994142015-04-24 Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech Rodet, F. Tasiemski, A. Boidin-Wichlacz, C. Van Camp, C. Vuillaume, C. Slomianny, C. Salzet, M. Sci Rep Article Unlike mammals, the CNS of the medicinal leech can regenerate damaged neurites, thus restoring neural functions after lesion. We previously demonstrated that the injured leech nerve cord is able to mount an immune response promoting the regenerative processes. Indeed neurons and microglia express sensing receptors like Hm-TLR1, a leech TLR ortholog, associated with chemokine release in response to a septic challenge or lesion. To gain insights into the TLR signaling pathways involved during these neuroimmune responses, members of the MyD88 family were investigated. In the present study, we report the characterization of Hm-MyD88 and Hm-SARM. The expression of their encoding gene was strongly regulated in leech CNS not only upon immune challenge but also during CNS repair, suggesting their involvement in both processes. This work also showed for the first time that differentiated neurons of the CNS could respond to LPS through a MyD88-dependent signalling pathway, while in mammals, studies describing the direct effect of LPS on neurons and the outcomes of such treatment are scarce and controversial. In the present study, we established that this PAMP induced the relocalization of Hm-MyD88 in isolated neurons. Nature Publishing Group 2015-04-16 /pmc/articles/PMC4399414/ /pubmed/25880897 http://dx.doi.org/10.1038/srep09624 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rodet, F.
Tasiemski, A.
Boidin-Wichlacz, C.
Van Camp, C.
Vuillaume, C.
Slomianny, C.
Salzet, M.
Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title_full Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title_fullStr Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title_full_unstemmed Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title_short Hm-MyD88 and Hm-SARM: Two key regulators of the neuroimmune system and neural repair in the medicinal leech
title_sort hm-myd88 and hm-sarm: two key regulators of the neuroimmune system and neural repair in the medicinal leech
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399414/
https://www.ncbi.nlm.nih.gov/pubmed/25880897
http://dx.doi.org/10.1038/srep09624
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