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CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently been developed. However, especially when multiple genetic manipulations are required, strain construction is still a time-consuming process. This study describes new CRISPR/Cas9-based approaches for easy, fast s...

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Detalles Bibliográficos
Autores principales:	Mans, Robert, van Rossum, Harmen M., Wijsman, Melanie, Backx, Antoon, Kuijpers, Niels G.A., van den Broek, Marcel, Daran-Lapujade, Pascale, Pronk, Jack T., van Maris, Antonius J.A., Daran, Jean-Marc G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399441/ https://www.ncbi.nlm.nih.gov/pubmed/25743786 http://dx.doi.org/10.1093/femsyr/fov004

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