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Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women

Group B streptococcus (GBS), which commonly colonizes the female genital tract and rectum, can cause infections in newborns with varying severity, possibly leading to death. The aim of the present study was to evaluate Hitchens-Pike-Todd-Hewitt (HPTH) medium performance for GBS screening in pregnant...

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Autores principales: de Melo, Simone Cristina Castanho Sabaini, Gavena, Angela Andréia França, Silva, Flávia Teixeira Ribeiro, Moreira, Ricardo Castanho, de Lima Scodro, Regiane Bertin, Cardoso, Rosilene Fressatti, Siqueira, Vera Lúcia Dias, de Pádua, Rúbia Andreia Faleiros, Carvalho, Maria Dalva de Barros, Pelloso, Sandra Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400012/
https://www.ncbi.nlm.nih.gov/pubmed/25881083
http://dx.doi.org/10.1371/journal.pone.0123988
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author de Melo, Simone Cristina Castanho Sabaini
Gavena, Angela Andréia França
Silva, Flávia Teixeira Ribeiro
Moreira, Ricardo Castanho
de Lima Scodro, Regiane Bertin
Cardoso, Rosilene Fressatti
Siqueira, Vera Lúcia Dias
de Pádua, Rúbia Andreia Faleiros
Carvalho, Maria Dalva de Barros
Pelloso, Sandra Marisa
author_facet de Melo, Simone Cristina Castanho Sabaini
Gavena, Angela Andréia França
Silva, Flávia Teixeira Ribeiro
Moreira, Ricardo Castanho
de Lima Scodro, Regiane Bertin
Cardoso, Rosilene Fressatti
Siqueira, Vera Lúcia Dias
de Pádua, Rúbia Andreia Faleiros
Carvalho, Maria Dalva de Barros
Pelloso, Sandra Marisa
author_sort de Melo, Simone Cristina Castanho Sabaini
collection PubMed
description Group B streptococcus (GBS), which commonly colonizes the female genital tract and rectum, can cause infections in newborns with varying severity, possibly leading to death. The aim of the present study was to evaluate Hitchens-Pike-Todd-Hewitt (HPTH) medium performance for GBS screening in pregnant women. A descriptive analytical cross-sectional study was performed with 556 pregnant women, of which 496 were at 35-37 weeks of gestation and 60 were at ≥ 38 weeks of gestation. The study was conducted from September 2011 to March 2014 in northern Paraná, Brazil. Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth. Of the 496 pregnant women at 35-37 weeks of gestation, 141 (28.4%) were positive for GBS, based on the combination of the three culture media and clinical specimens. The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth. Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS. These results demonstrate that HPTH medium and SBA were more sensitive than Todd-Hewitt enrichment broth for GBS screening in pregnant women and good GBS recovery in culture, indicating that the two media should be used together for vaginal and anorectal specimens.
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spelling pubmed-44000122015-04-21 Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women de Melo, Simone Cristina Castanho Sabaini Gavena, Angela Andréia França Silva, Flávia Teixeira Ribeiro Moreira, Ricardo Castanho de Lima Scodro, Regiane Bertin Cardoso, Rosilene Fressatti Siqueira, Vera Lúcia Dias de Pádua, Rúbia Andreia Faleiros Carvalho, Maria Dalva de Barros Pelloso, Sandra Marisa PLoS One Research Article Group B streptococcus (GBS), which commonly colonizes the female genital tract and rectum, can cause infections in newborns with varying severity, possibly leading to death. The aim of the present study was to evaluate Hitchens-Pike-Todd-Hewitt (HPTH) medium performance for GBS screening in pregnant women. A descriptive analytical cross-sectional study was performed with 556 pregnant women, of which 496 were at 35-37 weeks of gestation and 60 were at ≥ 38 weeks of gestation. The study was conducted from September 2011 to March 2014 in northern Paraná, Brazil. Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth. Of the 496 pregnant women at 35-37 weeks of gestation, 141 (28.4%) were positive for GBS, based on the combination of the three culture media and clinical specimens. The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth. Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS. These results demonstrate that HPTH medium and SBA were more sensitive than Todd-Hewitt enrichment broth for GBS screening in pregnant women and good GBS recovery in culture, indicating that the two media should be used together for vaginal and anorectal specimens. Public Library of Science 2015-04-16 /pmc/articles/PMC4400012/ /pubmed/25881083 http://dx.doi.org/10.1371/journal.pone.0123988 Text en © 2015 de Melo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Melo, Simone Cristina Castanho Sabaini
Gavena, Angela Andréia França
Silva, Flávia Teixeira Ribeiro
Moreira, Ricardo Castanho
de Lima Scodro, Regiane Bertin
Cardoso, Rosilene Fressatti
Siqueira, Vera Lúcia Dias
de Pádua, Rúbia Andreia Faleiros
Carvalho, Maria Dalva de Barros
Pelloso, Sandra Marisa
Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title_full Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title_fullStr Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title_full_unstemmed Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title_short Performance of Hitchens-Pike-Todd-Hewitt Medium for Group B Streptococcus Screening in Pregnant Women
title_sort performance of hitchens-pike-todd-hewitt medium for group b streptococcus screening in pregnant women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400012/
https://www.ncbi.nlm.nih.gov/pubmed/25881083
http://dx.doi.org/10.1371/journal.pone.0123988
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