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Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings

BACKGROUND: Because of the optical features of heart tissue, optical and electrical action potentials are only moderately associated, especially when near-infrared dyes are used in optical mapping (OM) studies. OBJECTIVE: By simultaneously recording transmural electrical action potentials (APs) and...

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Autores principales: Mačianskienė, Regina, Martišienė, Irma, Navalinskas, Antanas, Vosyliūtė, Rūta, Treinys, Rimantas, Vaidelytė, Birutė, Benetis, Rimantas, Jurevičius, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400155/
https://www.ncbi.nlm.nih.gov/pubmed/25881157
http://dx.doi.org/10.1371/journal.pone.0123050
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author Mačianskienė, Regina
Martišienė, Irma
Navalinskas, Antanas
Vosyliūtė, Rūta
Treinys, Rimantas
Vaidelytė, Birutė
Benetis, Rimantas
Jurevičius, Jonas
author_facet Mačianskienė, Regina
Martišienė, Irma
Navalinskas, Antanas
Vosyliūtė, Rūta
Treinys, Rimantas
Vaidelytė, Birutė
Benetis, Rimantas
Jurevičius, Jonas
author_sort Mačianskienė, Regina
collection PubMed
description BACKGROUND: Because of the optical features of heart tissue, optical and electrical action potentials are only moderately associated, especially when near-infrared dyes are used in optical mapping (OM) studies. OBJECTIVE: By simultaneously recording transmural electrical action potentials (APs) and optical action potentials (OAPs), we aimed to evaluate the contributions of both electrical and optical influences to the shape of the OAP upstroke. METHODS AND RESULTS: A standard glass microelectrode and OM, using an near-infrared fluorescent dye (di-4-ANBDQBS), were used to simultaneously record transmural APs and OAPs in a Langendorff-perfused rabbit heart during atrial, endocardial, and epicardial pacing. The actual profile of the transmural AP upstroke across the LV wall, together with the OAP upstroke, allowed for calculations of the probing-depth constant (k ~2.1 mm, n = 24) of the fluorescence measurements. In addition, the transmural AP recordings aided the quantitative evaluation of the influences of depth-weighted and lateral-scattering components on the OAP upstroke. These components correspond to the components of the propagating electrical wave that are transmural and parallel to the epicardium. The calculated mean values for the depth-weighted and lateral-scattering components, whose sum comprises the OAP upstroke, were (in ms) 10.18 ± 0.62 and 0.0 ± 0.56 for atrial stimulation, 9.37 ± 1.12 and 3.01 ± 1.30 for endocardial stimulation, and 6.09 ± 0.79 and 8.16 ± 0.98 for epicardial stimulation; (n = 8 for each). For this dye, 90% of the collected fluorescence originated up to 4.83 ± 0.18 mm (n = 24) from the epicardium. CONCLUSIONS: The co-registration of OM and transmural microelectrode APs enabled the probing depth of fluorescence measurements to be calculated and the OAP upstroke to be divided into two components (depth-weighted and lateral-scattering), and it also allowed the relative strengths of their effects on the shape of the OAP upstroke to be evaluated.
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spelling pubmed-44001552015-04-21 Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings Mačianskienė, Regina Martišienė, Irma Navalinskas, Antanas Vosyliūtė, Rūta Treinys, Rimantas Vaidelytė, Birutė Benetis, Rimantas Jurevičius, Jonas PLoS One Research Article BACKGROUND: Because of the optical features of heart tissue, optical and electrical action potentials are only moderately associated, especially when near-infrared dyes are used in optical mapping (OM) studies. OBJECTIVE: By simultaneously recording transmural electrical action potentials (APs) and optical action potentials (OAPs), we aimed to evaluate the contributions of both electrical and optical influences to the shape of the OAP upstroke. METHODS AND RESULTS: A standard glass microelectrode and OM, using an near-infrared fluorescent dye (di-4-ANBDQBS), were used to simultaneously record transmural APs and OAPs in a Langendorff-perfused rabbit heart during atrial, endocardial, and epicardial pacing. The actual profile of the transmural AP upstroke across the LV wall, together with the OAP upstroke, allowed for calculations of the probing-depth constant (k ~2.1 mm, n = 24) of the fluorescence measurements. In addition, the transmural AP recordings aided the quantitative evaluation of the influences of depth-weighted and lateral-scattering components on the OAP upstroke. These components correspond to the components of the propagating electrical wave that are transmural and parallel to the epicardium. The calculated mean values for the depth-weighted and lateral-scattering components, whose sum comprises the OAP upstroke, were (in ms) 10.18 ± 0.62 and 0.0 ± 0.56 for atrial stimulation, 9.37 ± 1.12 and 3.01 ± 1.30 for endocardial stimulation, and 6.09 ± 0.79 and 8.16 ± 0.98 for epicardial stimulation; (n = 8 for each). For this dye, 90% of the collected fluorescence originated up to 4.83 ± 0.18 mm (n = 24) from the epicardium. CONCLUSIONS: The co-registration of OM and transmural microelectrode APs enabled the probing depth of fluorescence measurements to be calculated and the OAP upstroke to be divided into two components (depth-weighted and lateral-scattering), and it also allowed the relative strengths of their effects on the shape of the OAP upstroke to be evaluated. Public Library of Science 2015-04-16 /pmc/articles/PMC4400155/ /pubmed/25881157 http://dx.doi.org/10.1371/journal.pone.0123050 Text en © 2015 Mačianskienė et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mačianskienė, Regina
Martišienė, Irma
Navalinskas, Antanas
Vosyliūtė, Rūta
Treinys, Rimantas
Vaidelytė, Birutė
Benetis, Rimantas
Jurevičius, Jonas
Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title_full Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title_fullStr Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title_full_unstemmed Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title_short Evaluation of Excitation Propagation in the Rabbit Heart: Optical Mapping and Transmural Microelectrode Recordings
title_sort evaluation of excitation propagation in the rabbit heart: optical mapping and transmural microelectrode recordings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400155/
https://www.ncbi.nlm.nih.gov/pubmed/25881157
http://dx.doi.org/10.1371/journal.pone.0123050
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