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Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration

Subcutaneous adipose tissue can be obtained for research during an elective, clinically indicated operation by standard surgical excision approaches and by needle aspiration in pure research settings. Whether measurements of inflammatory markers and cells made in tissue collected these two different...

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Detalles Bibliográficos
Autores principales: Santosa, Sylvia, Swain, James, Tchkonia, Tamara, Kirkland, James L, Jensen, Michael D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400182/
https://www.ncbi.nlm.nih.gov/pubmed/25319743
http://dx.doi.org/10.1038/ijo.2014.185
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author Santosa, Sylvia
Swain, James
Tchkonia, Tamara
Kirkland, James L
Jensen, Michael D
author_facet Santosa, Sylvia
Swain, James
Tchkonia, Tamara
Kirkland, James L
Jensen, Michael D
author_sort Santosa, Sylvia
collection PubMed
description Subcutaneous adipose tissue can be obtained for research during an elective, clinically indicated operation by standard surgical excision approaches and by needle aspiration in pure research settings. Whether measurements of inflammatory markers and cells made in tissue collected these two different ways are comparable is debatable. We sought to determine whether these two techniques yield systematically different results for measurements of inflammation, cellular senescence, and adipose tissue composition. Twelve subjects undergoing surgery participated. At the time of surgery abdominal subcutaneous adipose tissue from adjacent sites was removed by excision and needle aspiration. Stromovascular cell composition (flow cytometry), the number of senescent cells (senescence-associated-β-galactosidase staining), and IL-6, IL-1, TNF-α, MCP1 mRNA (RT-PCR) were measured in each sample. We found no statistically significant differences between the two sample collection approaches for any of the parameters measured. We conclude that these two methods of obtaining adipose tissue do not systematically differ in the results of cytokine mRNA content, cellular senescence, or stromovascular cell composition.
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spelling pubmed-44001822015-11-01 Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration Santosa, Sylvia Swain, James Tchkonia, Tamara Kirkland, James L Jensen, Michael D Int J Obes (Lond) Article Subcutaneous adipose tissue can be obtained for research during an elective, clinically indicated operation by standard surgical excision approaches and by needle aspiration in pure research settings. Whether measurements of inflammatory markers and cells made in tissue collected these two different ways are comparable is debatable. We sought to determine whether these two techniques yield systematically different results for measurements of inflammation, cellular senescence, and adipose tissue composition. Twelve subjects undergoing surgery participated. At the time of surgery abdominal subcutaneous adipose tissue from adjacent sites was removed by excision and needle aspiration. Stromovascular cell composition (flow cytometry), the number of senescent cells (senescence-associated-β-galactosidase staining), and IL-6, IL-1, TNF-α, MCP1 mRNA (RT-PCR) were measured in each sample. We found no statistically significant differences between the two sample collection approaches for any of the parameters measured. We conclude that these two methods of obtaining adipose tissue do not systematically differ in the results of cytokine mRNA content, cellular senescence, or stromovascular cell composition. 2014-10-16 2015-05 /pmc/articles/PMC4400182/ /pubmed/25319743 http://dx.doi.org/10.1038/ijo.2014.185 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Santosa, Sylvia
Swain, James
Tchkonia, Tamara
Kirkland, James L
Jensen, Michael D
Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title_full Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title_fullStr Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title_full_unstemmed Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title_short Inflammatory Characteristics of Adipose Tissue Collected by Surgical Excision vs. Needle Aspiration
title_sort inflammatory characteristics of adipose tissue collected by surgical excision vs. needle aspiration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400182/
https://www.ncbi.nlm.nih.gov/pubmed/25319743
http://dx.doi.org/10.1038/ijo.2014.185
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