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Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response
Both viral infection and DNA transfection expose single-stranded or double-stranded DNA to the cytoplasm of mammalian cells. Recognition of cytosolic DNA activates a series of cellular responses, including induction of pro-inflammatory genes such as type I interferon through the well-known cGAS-STIN...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400389/ https://www.ncbi.nlm.nih.gov/pubmed/25892965 http://dx.doi.org/10.7150/ijbs.10945 |
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author | Zheng, Min Xie, Linna Liang, Yaoji Wu, Suqin Xu, Huijuan Zhang, Yuedong Liu, Hekun Lin, Dexin Han, Jiahuai Lu, Kunping |
author_facet | Zheng, Min Xie, Linna Liang, Yaoji Wu, Suqin Xu, Huijuan Zhang, Yuedong Liu, Hekun Lin, Dexin Han, Jiahuai Lu, Kunping |
author_sort | Zheng, Min |
collection | PubMed |
description | Both viral infection and DNA transfection expose single-stranded or double-stranded DNA to the cytoplasm of mammalian cells. Recognition of cytosolic DNA activates a series of cellular responses, including induction of pro-inflammatory genes such as type I interferon through the well-known cGAS-STING pathway. Here we show for the first time that intracellular administration of either single or double stranded interferon stimulating DNA (ISD), but not poly(dA) suppresses cell growth in many different cell types. Suppression of cell growth by cytosolic DNA is cGAS/STING independent and associated with inhibition of glucose metabolism, ATP depletion and subsequent cellular energy stress responses including activation of AMPK and inactivation of mTORC1. Our results suggest that in concert with but independent of innate immune response, recognition of cytosolic DNA induced cellular energy stress potentially functions as a metabolic barrier to viral replication. |
format | Online Article Text |
id | pubmed-4400389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-44003892015-04-17 Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response Zheng, Min Xie, Linna Liang, Yaoji Wu, Suqin Xu, Huijuan Zhang, Yuedong Liu, Hekun Lin, Dexin Han, Jiahuai Lu, Kunping Int J Biol Sci Research Paper Both viral infection and DNA transfection expose single-stranded or double-stranded DNA to the cytoplasm of mammalian cells. Recognition of cytosolic DNA activates a series of cellular responses, including induction of pro-inflammatory genes such as type I interferon through the well-known cGAS-STING pathway. Here we show for the first time that intracellular administration of either single or double stranded interferon stimulating DNA (ISD), but not poly(dA) suppresses cell growth in many different cell types. Suppression of cell growth by cytosolic DNA is cGAS/STING independent and associated with inhibition of glucose metabolism, ATP depletion and subsequent cellular energy stress responses including activation of AMPK and inactivation of mTORC1. Our results suggest that in concert with but independent of innate immune response, recognition of cytosolic DNA induced cellular energy stress potentially functions as a metabolic barrier to viral replication. Ivyspring International Publisher 2015-04-05 /pmc/articles/PMC4400389/ /pubmed/25892965 http://dx.doi.org/10.7150/ijbs.10945 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Zheng, Min Xie, Linna Liang, Yaoji Wu, Suqin Xu, Huijuan Zhang, Yuedong Liu, Hekun Lin, Dexin Han, Jiahuai Lu, Kunping Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title | Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title_full | Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title_fullStr | Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title_full_unstemmed | Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title_short | Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response |
title_sort | recognition of cytosolic dna attenuates glucose metabolism and induces ampk mediated energy stress response |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400389/ https://www.ncbi.nlm.nih.gov/pubmed/25892965 http://dx.doi.org/10.7150/ijbs.10945 |
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