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Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule

Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approac...

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Autores principales: Honeth, Gabriella, Schiavinotto, Tommaso, Vaggi, Federico, Marlow, Rebecca, Kanno, Tokuwa, Shinomiya, Ireneusz, Lombardi, Sara, Buchupalli, Bharath, Graham, Rosalind, Gazinska, Patrycja, Ramalingam, Vernie, Burchell, Joy, Purushotham, Anand D., Pinder, Sarah E., Csikasz-Nagy, Attila, Dontu, Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400614/
https://www.ncbi.nlm.nih.gov/pubmed/25818813
http://dx.doi.org/10.1016/j.stemcr.2015.02.013
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author Honeth, Gabriella
Schiavinotto, Tommaso
Vaggi, Federico
Marlow, Rebecca
Kanno, Tokuwa
Shinomiya, Ireneusz
Lombardi, Sara
Buchupalli, Bharath
Graham, Rosalind
Gazinska, Patrycja
Ramalingam, Vernie
Burchell, Joy
Purushotham, Anand D.
Pinder, Sarah E.
Csikasz-Nagy, Attila
Dontu, Gabriela
author_facet Honeth, Gabriella
Schiavinotto, Tommaso
Vaggi, Federico
Marlow, Rebecca
Kanno, Tokuwa
Shinomiya, Ireneusz
Lombardi, Sara
Buchupalli, Bharath
Graham, Rosalind
Gazinska, Patrycja
Ramalingam, Vernie
Burchell, Joy
Purushotham, Anand D.
Pinder, Sarah E.
Csikasz-Nagy, Attila
Dontu, Gabriela
author_sort Honeth, Gabriella
collection PubMed
description Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.
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spelling pubmed-44006142015-04-22 Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule Honeth, Gabriella Schiavinotto, Tommaso Vaggi, Federico Marlow, Rebecca Kanno, Tokuwa Shinomiya, Ireneusz Lombardi, Sara Buchupalli, Bharath Graham, Rosalind Gazinska, Patrycja Ramalingam, Vernie Burchell, Joy Purushotham, Anand D. Pinder, Sarah E. Csikasz-Nagy, Attila Dontu, Gabriela Stem Cell Reports Resource Characterization of normal breast stem cells is important for understanding their role in breast development and in breast cancer. However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known. In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals. Comparing predictions of these models with immunohistochemical analysis of tissue sections for candidate stem cell markers, we defined distinct areas where stem cells reside in the mammary lobule. An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of nulliparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers. Elsevier 2015-03-26 /pmc/articles/PMC4400614/ /pubmed/25818813 http://dx.doi.org/10.1016/j.stemcr.2015.02.013 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Resource
Honeth, Gabriella
Schiavinotto, Tommaso
Vaggi, Federico
Marlow, Rebecca
Kanno, Tokuwa
Shinomiya, Ireneusz
Lombardi, Sara
Buchupalli, Bharath
Graham, Rosalind
Gazinska, Patrycja
Ramalingam, Vernie
Burchell, Joy
Purushotham, Anand D.
Pinder, Sarah E.
Csikasz-Nagy, Attila
Dontu, Gabriela
Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title_full Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title_fullStr Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title_full_unstemmed Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title_short Models of Breast Morphogenesis Based on Localization of Stem Cells in the Developing Mammary Lobule
title_sort models of breast morphogenesis based on localization of stem cells in the developing mammary lobule
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4400614/
https://www.ncbi.nlm.nih.gov/pubmed/25818813
http://dx.doi.org/10.1016/j.stemcr.2015.02.013
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