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Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers

BACKGROUND: Diarrheal diseases can be caused by viral, bacterial and parasitic infections. This paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in Tunisian children less than five years of age. METHODS: Overall, 124 diarrhoeal stools were collected f...

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Autores principales: BEN SALEM-BEN NEJMA, Imen, HASSINE ZAAFRANE, Mouna, HASSINE, Fredj, SDIRI-LOULIZI, Khira, BEN SAID, Moncef, AOUNI, Mahjoub, MZOUGHI, Ridha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401059/
https://www.ncbi.nlm.nih.gov/pubmed/25909062
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author BEN SALEM-BEN NEJMA, Imen
HASSINE ZAAFRANE, Mouna
HASSINE, Fredj
SDIRI-LOULIZI, Khira
BEN SAID, Moncef
AOUNI, Mahjoub
MZOUGHI, Ridha
author_facet BEN SALEM-BEN NEJMA, Imen
HASSINE ZAAFRANE, Mouna
HASSINE, Fredj
SDIRI-LOULIZI, Khira
BEN SAID, Moncef
AOUNI, Mahjoub
MZOUGHI, Ridha
author_sort BEN SALEM-BEN NEJMA, Imen
collection PubMed
description BACKGROUND: Diarrheal diseases can be caused by viral, bacterial and parasitic infections. This paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in Tunisian children less than five years of age. METHODS: Overall, 124 diarrhoeal stools were collected from patients suffering from acute diarrhea and 54 stool samples from healthy children. All stools were examined for the presence of enteric pathogens. RESULTS: In diarrheagenic children, 107 pathogenic bacteria were isolated (12 Salmonella spp. (9.7%) and 95 diarrheagenic Escherichia coli strains (76.6%): 29 enteroaggregative E.coli (EAEC) (23.4%), 15 enteroinvasive E.coli (EIEC) (12.1%), 17 enteropathogenic E.coli (EPEC) (13.7%), 26 enterotoxigenic E.coli (ETEC) (21%) and 2 enterohemoragic E.coli (EHEC) (1.6%). However, in the control group, 23 pathogenic E.coli strains were isolated (42.6%): 8 EAEC (14.8%), 12 EIEC (22.2%) and 3 EPEC (5.5%). Among diarrheagenic E.coli (DEC), only ETEC strains were significantly recovered from diarrheagenic children than from healthy controls (P < 0.0003). Group A rotavirus was identified in 33.9% (n=42) of diarrheagenic children and in 11.1% among the control group (n=6). Concerning norovirus, 8.9% (n=11) of the samples collected from diarrheagenic children and 9.2% (n=5) from the control group were positive. The prevalence of rotaviruses and Salmonella spp were also significantly higher in patients with diarrhea than in controls (P = 0.002 and P < 0.019, respectively). Finally, enteropathogenic parasites (Entamoeba coli and cryptosporidium Oocystes) were isolated from 4.8% and 9.2% of diarrheagenic and control children, respectively. CONCLUSION: These results provide baseline data about the relative importance of different enteropathogens in Tunisian children.
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spelling pubmed-44010592015-04-23 Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers BEN SALEM-BEN NEJMA, Imen HASSINE ZAAFRANE, Mouna HASSINE, Fredj SDIRI-LOULIZI, Khira BEN SAID, Moncef AOUNI, Mahjoub MZOUGHI, Ridha Iran J Public Health Original Article BACKGROUND: Diarrheal diseases can be caused by viral, bacterial and parasitic infections. This paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in Tunisian children less than five years of age. METHODS: Overall, 124 diarrhoeal stools were collected from patients suffering from acute diarrhea and 54 stool samples from healthy children. All stools were examined for the presence of enteric pathogens. RESULTS: In diarrheagenic children, 107 pathogenic bacteria were isolated (12 Salmonella spp. (9.7%) and 95 diarrheagenic Escherichia coli strains (76.6%): 29 enteroaggregative E.coli (EAEC) (23.4%), 15 enteroinvasive E.coli (EIEC) (12.1%), 17 enteropathogenic E.coli (EPEC) (13.7%), 26 enterotoxigenic E.coli (ETEC) (21%) and 2 enterohemoragic E.coli (EHEC) (1.6%). However, in the control group, 23 pathogenic E.coli strains were isolated (42.6%): 8 EAEC (14.8%), 12 EIEC (22.2%) and 3 EPEC (5.5%). Among diarrheagenic E.coli (DEC), only ETEC strains were significantly recovered from diarrheagenic children than from healthy controls (P < 0.0003). Group A rotavirus was identified in 33.9% (n=42) of diarrheagenic children and in 11.1% among the control group (n=6). Concerning norovirus, 8.9% (n=11) of the samples collected from diarrheagenic children and 9.2% (n=5) from the control group were positive. The prevalence of rotaviruses and Salmonella spp were also significantly higher in patients with diarrhea than in controls (P = 0.002 and P < 0.019, respectively). Finally, enteropathogenic parasites (Entamoeba coli and cryptosporidium Oocystes) were isolated from 4.8% and 9.2% of diarrheagenic and control children, respectively. CONCLUSION: These results provide baseline data about the relative importance of different enteropathogens in Tunisian children. Tehran University of Medical Sciences 2014-07 /pmc/articles/PMC4401059/ /pubmed/25909062 Text en Copyright © Iranian Public Health Association & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
BEN SALEM-BEN NEJMA, Imen
HASSINE ZAAFRANE, Mouna
HASSINE, Fredj
SDIRI-LOULIZI, Khira
BEN SAID, Moncef
AOUNI, Mahjoub
MZOUGHI, Ridha
Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title_full Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title_fullStr Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title_full_unstemmed Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title_short Etiology of Acute Diarrhea in Tunisian Children with Emphasis on Diarrheagenic Escherichia coli: Prevalence and Identification of E. coli Virulence Markers
title_sort etiology of acute diarrhea in tunisian children with emphasis on diarrheagenic escherichia coli: prevalence and identification of e. coli virulence markers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401059/
https://www.ncbi.nlm.nih.gov/pubmed/25909062
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