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Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin

Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (...

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Detalles Bibliográficos
Autores principales: Wagner, W, Wehrmann, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401296/
https://www.ncbi.nlm.nih.gov/pubmed/18205704
http://dx.doi.org/10.1111/j.1582-4934.2007.00037.x
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author Wagner, W
Wehrmann, M
author_facet Wagner, W
Wehrmann, M
author_sort Wagner, W
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description Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) α, TGFβ(1), −β(2) and −β(3), IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-β(1) as therapeutic targets in efforts to optimize wound healing in the adult organism.
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spelling pubmed-44012962015-04-27 Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin Wagner, W Wehrmann, M J Cell Mol Med Articles Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) α, TGFβ(1), −β(2) and −β(3), IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-β(1) as therapeutic targets in efforts to optimize wound healing in the adult organism. Blackwell Publishing Ltd 2007-11 2007-06-24 /pmc/articles/PMC4401296/ /pubmed/18205704 http://dx.doi.org/10.1111/j.1582-4934.2007.00037.x Text en
spellingShingle Articles
Wagner, W
Wehrmann, M
Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title_full Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title_fullStr Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title_full_unstemmed Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title_short Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
title_sort differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401296/
https://www.ncbi.nlm.nih.gov/pubmed/18205704
http://dx.doi.org/10.1111/j.1582-4934.2007.00037.x
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