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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Described in this report is a qRT-PCR assay for the analysis of seventeen human IFN subtypes in a 384-well plate format that incorporates highly specific locked nucleic acid (LNA) and molecular beacon (MB) probes, transcript standards, automated multichannel pipetting, and plate drying. Determining...
Autores principales: | Renn, Lynnsey A., Theisen, Terence C., Navarro, Maria B., Mane, Viraj P., Schramm, Lynnsie M., Kirschman, Kevin D., Fabozzi, Giulia, Hillyer, Philippa, Puig, Montserrat, Verthelyi, Daniela, Rabin, Ronald L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401384/ https://www.ncbi.nlm.nih.gov/pubmed/25867042 http://dx.doi.org/10.3791/52650 |
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