Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence (NIRF) xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies. First we describe how to generate subcutaneous tumors in mice, using antigen-n...

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Detalles Bibliográficos
Autores principales: Bannas, Peter, Lenz, Alexander, Kunick, Valentin, Fumey, William, Rissiek, Björn, Schmid, Joanna, Haag, Friedrich, Leingärtner, Axel, Trepel, Martin, Adam, Gerhard, Koch-Nolte, Friedrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401403/
https://www.ncbi.nlm.nih.gov/pubmed/25867711
http://dx.doi.org/10.3791/52462

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401403/
https://www.ncbi.nlm.nih.gov/pubmed/25867711
http://dx.doi.org/10.3791/52462

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