Cargando…

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence (NIRF) xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies. First we describe how to generate subcutaneous tumors in mice, using antigen-n...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bannas, Peter, Lenz, Alexander, Kunick, Valentin, Fumey, William, Rissiek, Björn, Schmid, Joanna, Haag, Friedrich, Leingärtner, Axel, Trepel, Martin, Adam, Gerhard, Koch-Nolte, Friedrich
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2015
Materias:	Medicine
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401403/ https://www.ncbi.nlm.nih.gov/pubmed/25867711 http://dx.doi.org/10.3791/52462

Ejemplares similares

Nanobodies effectively modulate the enzymatic activity of CD38 and allow specific imaging of CD38(+) tumors in mouse models in vivo
por: Fumey, William, et al.
Publicado: (2017)

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics
por: Bannas, Peter, et al.
Publicado: (2017)

CD38-specific nanobodies allow in vivo imaging of multiple myeloma under daratumumab therapy
por: Pape, Luca Julius, et al.
Publicado: (2022)

Nanobodies as modulators of inflammation: potential applications for acute brain injury
por: Rissiek, Björn, et al.
Publicado: (2014)

Half-Life Extended Nanobody-Based CD38-Specific Bispecific Killercell Engagers Induce Killing of Multiple Myeloma Cells
por: Hambach, Julia, et al.
Publicado: (2022)

Nanobody-based CD38-specific heavy chain antibodies induce killing of multiple myeloma and other hematological malignancies
por: Schriewer, Levin, et al.
Publicado: (2020)

Targeting multiple myeloma with nanobody-based heavy chain antibodies, bispecific killer cell engagers, chimeric antigen receptors, and nanobody-displaying AAV vectors
por: Hambach, Julia, et al.
Publicado: (2022)

Evaluation of nanobody-based biologics targeting purinergic checkpoints in tumor models in vivo
por: Demeules, Mélanie, et al.
Publicado: (2022)

Daratumumab and Nanobody-Based Heavy Chain Antibodies Inhibit the ADPR Cyclase but not the NAD(+) Hydrolase Activity of CD38-Expressing Multiple Myeloma Cells
por: Baum, Natalie, et al.
Publicado: (2020)

Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope
por: Shrivastav, Manisha, et al.
Publicado: (2018)

Nanobody-Based Biologics for Modulating Purinergic Signaling in Inflammation and Immunity
por: Menzel, Stephan, et al.
Publicado: (2018)

Nanobody-Enhanced Targeting of AAV Gene Therapy Vectors
por: Eichhoff, Anna Marei, et al.
Publicado: (2019)

Correction: Validation of near infrared fluorescence (NIRF) probes in vivo with dual laser NIRF endoscope
por: Shrivastav, Manisha, et al.
Publicado: (2019)

Current Approaches and Future Perspectives for Nanobodies in Stroke Diagnostic and Therapy
por: Jank, Larissa, et al.
Publicado: (2019)

Effective targeting of microglial P2X7 following intracerebroventricular delivery of nanobodies and nanobody-encoding AAVs
por: Pinto-Espinoza, Carolina, et al.
Publicado: (2022)

P2X7 on Mouse T Cells: One Channel, Many Functions
por: Rissiek, Björn, et al.
Publicado: (2015)

CD38-Specific Biparatopic Heavy Chain Antibodies Display Potent Complement-Dependent Cytotoxicity Against Multiple Myeloma Cells
por: Schütze, Kerstin, et al.
Publicado: (2018)

Nanobodies: new avenue to treat kidney disease
por: Wanner, Nicola, et al.
Publicado: (2021)

Targeting CD38-Expressing Multiple Myeloma and Burkitt Lymphoma Cells In Vitro with Nanobody-Based Chimeric Antigen Receptors (Nb-CARs)
por: Hambach, Julia, et al.
Publicado: (2020)

In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells
por: Rissiek, Björn, et al.
Publicado: (2018)

Tuning IL-2 signaling by ADP-ribosylation of CD25
por: Teege, Sophie, et al.
Publicado: (2015)

Generation and Characterization of Specific Monoclonal Antibodies and Nanobodies Directed Against the ATP-Gated Channel P2X4
por: Bergmann, Philine, et al.
Publicado: (2019)

Perspectives for the Development of CD38-Specific Heavy Chain Antibodies as Therapeutics for Multiple Myeloma
por: Bannas, Peter, et al.
Publicado: (2018)

A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation
por: Eden, Thomas, et al.
Publicado: (2018)

ADP-Ribosylation Regulates the Signaling Function of IFN-γ
por: Menzel, Stephan, et al.
Publicado: (2021)

NIRF-Molecular Imaging with Synovial Macrophages-Targeting Vsig4 Nanobody for Disease Monitoring in a Mouse Model of Arthritis
por: Zheng, Fang, et al.
Publicado: (2019)

Circulating Tumor Cell Detection and Capture by Photoacoustic Flow Cytometry in Vivo and ex Vivo
por: Galanzha, Ekaterina I., et al.
Publicado: (2013)

Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry
por: Wirjanata, Grennady, et al.
Publicado: (2015)

Origin, distribution, and function of three frequent coding polymorphisms in the gene for the human P2X7 ion channel
por: Schäfer, Waldemar, et al.
Publicado: (2022)

Evaluation of P2X7 Receptor Function in Tumor Contexts Using rAAV Vector and Nanobodies (AAVnano)
por: Demeules, Mélanie, et al.
Publicado: (2020)

The Anti-ADAMTS-5 Nanobody(®) M6495 Protects Cartilage Degradation Ex Vivo
por: Siebuhr, Anne Sofie, et al.
Publicado: (2020)

Extracellular NAD and ATP: Partners in immune cell modulation
por: Haag, Friedrich, et al.
Publicado: (2007)

A Methodological Approach Using rAAV Vectors Encoding Nanobody-Based Biologics to Evaluate ARTC2.2 and P2X7 In Vivo
por: Gondé, Henri, et al.
Publicado: (2021)

Tri-functional platform for construction of modular antibody fragments for in vivo(18)F-PET or NIRF molecular imaging
por: Gamache, Raymond F., et al.
Publicado: (2020)

Extracellular NAD(+) shapes the Foxp3(+) regulatory T cell compartment through the ART2–P2X7 pathway
por: Hubert, Sandra, et al.
Publicado: (2010)

In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodies
por: Hammarén, Milka Marjut, et al.
Publicado: (2023)

Flow cytometry-based ex vivo murine NK cell cytotoxicity assay
por: Wong, Pamela, et al.
Publicado: (2021)

CD39 is upregulated during activation of mouse and human T cells and attenuates the immune response to Listeria monocytogenes
por: Raczkowski, Friederike, et al.
Publicado: (2018)

Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT
por: Unger, Mandy, et al.
Publicado: (2015)

Glycan-Based Near-infrared Fluorescent (NIRF) Imaging of Gastrointestinal Tumors: a Preclinical Proof-of-Concept In Vivo Study
por: Houvast, Ruben D., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_32v7ekgl9jvoap744pfuti4ckt