Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly

Here we report the crystal structures of the Thermus thermophilus ribosome at 2.3-2.5Å-resolution, which have enabled a comprehensive modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior st...

Descripción completa

Detalles Bibliográficos
Autores principales: Polikanov, Yury S., Melnikov, Sergey V., Söll, Dieter, Steitz, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401423/
https://www.ncbi.nlm.nih.gov/pubmed/25775268
http://dx.doi.org/10.1038/nsmb.2992
Descripción
Sumario:Here we report the crystal structures of the Thermus thermophilus ribosome at 2.3-2.5Å-resolution, which have enabled a comprehensive modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields the most complete atomic model of bacterial ribosome.

Ejemplares similares