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Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains

Astroviruses are a common cause of gastroenteritis in children worldwide. These viruses can also cause infection in a range of domestic and wild animal species. Canine astrovirus (CaAstV) was first identified in the USA, and has since been reported in dogs from Europe, the Far East and South America...

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Autores principales: Caddy, Sarah L., Goodfellow, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401448/
https://www.ncbi.nlm.nih.gov/pubmed/25818578
http://dx.doi.org/10.1016/j.vetmic.2015.03.011
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author Caddy, Sarah L.
Goodfellow, Ian
author_facet Caddy, Sarah L.
Goodfellow, Ian
author_sort Caddy, Sarah L.
collection PubMed
description Astroviruses are a common cause of gastroenteritis in children worldwide. These viruses can also cause infection in a range of domestic and wild animal species. Canine astrovirus (CaAstV) was first identified in the USA, and has since been reported in dogs from Europe, the Far East and South America. We sought to determine whether CaAstV is circulating in the UK dog population, and to characterise any identified strains. Stool samples were collected from pet dogs in the UK with and without gastroenteritis, and samples were screened for CaAstV by qPCR. Four CaAstV positive samples were identified from dogs with gastroenteritis (4/67, 6.0%), whereas no samples from healthy dogs were positive (p < 0.001). Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains. The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein. This is the first report describing the circulation of CaAstV in UK dogs with clinical signs of gastroenteritis, and the first description of the full-length genomes of two CaAstV strains.
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spelling pubmed-44014482015-05-15 Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains Caddy, Sarah L. Goodfellow, Ian Vet Microbiol Article Astroviruses are a common cause of gastroenteritis in children worldwide. These viruses can also cause infection in a range of domestic and wild animal species. Canine astrovirus (CaAstV) was first identified in the USA, and has since been reported in dogs from Europe, the Far East and South America. We sought to determine whether CaAstV is circulating in the UK dog population, and to characterise any identified strains. Stool samples were collected from pet dogs in the UK with and without gastroenteritis, and samples were screened for CaAstV by qPCR. Four CaAstV positive samples were identified from dogs with gastroenteritis (4/67, 6.0%), whereas no samples from healthy dogs were positive (p < 0.001). Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains. The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein. This is the first report describing the circulation of CaAstV in UK dogs with clinical signs of gastroenteritis, and the first description of the full-length genomes of two CaAstV strains. The Authors. Published by Elsevier B.V. 2015-05-15 2015-03-19 /pmc/articles/PMC4401448/ /pubmed/25818578 http://dx.doi.org/10.1016/j.vetmic.2015.03.011 Text en © 2015 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Caddy, Sarah L.
Goodfellow, Ian
Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title_full Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title_fullStr Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title_full_unstemmed Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title_short Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains
title_sort complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of uk strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401448/
https://www.ncbi.nlm.nih.gov/pubmed/25818578
http://dx.doi.org/10.1016/j.vetmic.2015.03.011
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