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Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation
PURPOSE: Cataract is the world-leading cause of blindness. In search for a new treatment of cataract we have found that the yellow discolouration of aged human lenses can be photobleached using a non-invasive, infra-red, femtosecond laser treatment. These results were presented in an earlier PlosOne...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401553/ https://www.ncbi.nlm.nih.gov/pubmed/25884924 http://dx.doi.org/10.1371/journal.pone.0123732 |
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author | Kessel, Line Larsen, Michael |
author_facet | Kessel, Line Larsen, Michael |
author_sort | Kessel, Line |
collection | PubMed |
description | PURPOSE: Cataract is the world-leading cause of blindness. In search for a new treatment of cataract we have found that the yellow discolouration of aged human lenses can be photobleached using a non-invasive, infra-red, femtosecond laser treatment. These results were presented in an earlier PlosOne publication. The objective of the study was to characterize the single-photon photobleaching action spectrum of the aged human lens in vitro. METHODS: Ninety-one human donor lenses were irradiated with continuous wave laser light at 375, 405, 420, 445, 457 or 473 nm. Photobleaching was monitored by photography and transmission measurements. RESULTS: The action spectrum peaked at 420 nm followed by, in order of decreasing effect, 445, 457, 473, 405 and 375 nm. Younger and less absorbent lenses showed smaller changes than older and more absorbent lenses. There was a dose-dependent increase in lens transmission with increasing laser irradiation. CONCLUSIONS: For a 75 year old lens an effect corresponding to elimination of 15 years or more of optical ageing was obtained. This study of the spectral characteristics and intensity needed to bleach the human lens with single-photon laser effects found an action-spectrum peak at 420 nm tailing gradually off toward longer wavelengths and more steeply toward shorter wavelengths. The results may be used to guide experiments with two-photon bleaching. |
format | Online Article Text |
id | pubmed-4401553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44015532015-04-21 Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation Kessel, Line Larsen, Michael PLoS One Research Article PURPOSE: Cataract is the world-leading cause of blindness. In search for a new treatment of cataract we have found that the yellow discolouration of aged human lenses can be photobleached using a non-invasive, infra-red, femtosecond laser treatment. These results were presented in an earlier PlosOne publication. The objective of the study was to characterize the single-photon photobleaching action spectrum of the aged human lens in vitro. METHODS: Ninety-one human donor lenses were irradiated with continuous wave laser light at 375, 405, 420, 445, 457 or 473 nm. Photobleaching was monitored by photography and transmission measurements. RESULTS: The action spectrum peaked at 420 nm followed by, in order of decreasing effect, 445, 457, 473, 405 and 375 nm. Younger and less absorbent lenses showed smaller changes than older and more absorbent lenses. There was a dose-dependent increase in lens transmission with increasing laser irradiation. CONCLUSIONS: For a 75 year old lens an effect corresponding to elimination of 15 years or more of optical ageing was obtained. This study of the spectral characteristics and intensity needed to bleach the human lens with single-photon laser effects found an action-spectrum peak at 420 nm tailing gradually off toward longer wavelengths and more steeply toward shorter wavelengths. The results may be used to guide experiments with two-photon bleaching. Public Library of Science 2015-04-17 /pmc/articles/PMC4401553/ /pubmed/25884924 http://dx.doi.org/10.1371/journal.pone.0123732 Text en © 2015 Kessel, Larsen http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kessel, Line Larsen, Michael Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title | Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title_full | Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title_fullStr | Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title_full_unstemmed | Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title_short | Action Spectrum for Photobleaching of Human Lenses by Short Wavelength Visible Irradiation |
title_sort | action spectrum for photobleaching of human lenses by short wavelength visible irradiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401553/ https://www.ncbi.nlm.nih.gov/pubmed/25884924 http://dx.doi.org/10.1371/journal.pone.0123732 |
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