Identification of New Genes Contributing to the Extreme Radioresistance of Deinococcus radiodurans Using a Tn5-Based Transposon Mutant Library

Here, we have developed an extremely efficient in vivo Tn5-based mutagenesis procedure to construct a Deinococcus radiodurans insertion mutant library subsequently screened for sensitivity to genotoxic agents such as γ and UV radiations or mitomycin C. The genes inactivated in radiosensitive mutants...

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Detalles Bibliográficos
Autores principales: Dulermo, Rémi, Onodera, Takefumi, Coste, Geneviève, Passot, Fanny, Dutertre, Murielle, Porteron, Martine, Confalonieri, Fabrice, Sommer, Suzanne, Pasternak, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4401554/
https://www.ncbi.nlm.nih.gov/pubmed/25884619
http://dx.doi.org/10.1371/journal.pone.0124358

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