Cargando…
Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age
We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2–1159 days) from patients of varying ages (26–62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and w...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402176/ https://www.ncbi.nlm.nih.gov/pubmed/25945096 http://dx.doi.org/10.1155/2015/146421 |
_version_ | 1782367235760717824 |
---|---|
author | Devitt, Sean M. Carter, Cynthia M. Dierov, Raia Weiss, Scott Gersch, Robert P. Percec, Ivona |
author_facet | Devitt, Sean M. Carter, Cynthia M. Dierov, Raia Weiss, Scott Gersch, Robert P. Percec, Ivona |
author_sort | Devitt, Sean M. |
collection | PubMed |
description | We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2–1159 days) from patients of varying ages (26–62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages. |
format | Online Article Text |
id | pubmed-4402176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44021762015-05-05 Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age Devitt, Sean M. Carter, Cynthia M. Dierov, Raia Weiss, Scott Gersch, Robert P. Percec, Ivona Stem Cells Int Research Article We examined cell isolation, viability, and growth in adipose-derived stem cells harvested from whole adipose tissue subject to different cryopreservation lengths (2–1159 days) from patients of varying ages (26–62 years). Subcutaneous abdominal adipose tissue was excised during abdominoplasties and was cryopreserved. The viability and number of adipose-derived stem cells isolated were measured after initial isolation and after 9, 18, and 28 days of growth. Data were analyzed with respect to cryopreservation duration and patient age. Significantly more viable cells were initially isolated from tissue cryopreserved <1 year than from tissue cryopreserved >2 years, irrespective of patient age. However, this difference did not persist with continued growth and there were no significant differences in cell viability or growth at subsequent time points with respect to cryopreservation duration or patient age. Mesenchymal stem cell markers were maintained in all cohorts tested throughout the duration of the study. Consequently, longer cryopreservation negatively impacts initial live adipose-derived stem cell isolation; however, this effect is neutralized with continued cell growth. Patient age does not significantly impact stem cell isolation, viability, or growth. Cryopreservation of adipose tissue is an effective long-term banking method for isolation of adipose-derived stem cells in patients of varying ages. Hindawi Publishing Corporation 2015 2015-04-05 /pmc/articles/PMC4402176/ /pubmed/25945096 http://dx.doi.org/10.1155/2015/146421 Text en Copyright © 2015 Sean M. Devitt et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Devitt, Sean M. Carter, Cynthia M. Dierov, Raia Weiss, Scott Gersch, Robert P. Percec, Ivona Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title | Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title_full | Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title_fullStr | Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title_full_unstemmed | Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title_short | Successful Isolation of Viable Adipose-Derived Stem Cells from Human Adipose Tissue Subject to Long-Term Cryopreservation: Positive Implications for Adult Stem Cell-Based Therapeutics in Patients of Advanced Age |
title_sort | successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402176/ https://www.ncbi.nlm.nih.gov/pubmed/25945096 http://dx.doi.org/10.1155/2015/146421 |
work_keys_str_mv | AT devittseanm successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage AT cartercynthiam successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage AT dierovraia successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage AT weissscott successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage AT gerschrobertp successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage AT percecivona successfulisolationofviableadiposederivedstemcellsfromhumanadiposetissuesubjecttolongtermcryopreservationpositiveimplicationsforadultstemcellbasedtherapeuticsinpatientsofadvancedage |