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Mechano-chemical kinetics of DNA replication: identification of the translocation step of a replicative DNA polymerase

During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers to study the translocation mechanism of individual bacteriophage Phi29 DNA polymerase...

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Detalles Bibliográficos
Autores principales:	Morin, José A., Cao, Francisco J., Lázaro, José M., Arias-Gonzalez, J. Ricardo, Valpuesta, José M., Carrascosa, José L., Salas, Margarita, Ibarra, Borja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Genome Integrity, Repair and Replication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402526/ https://www.ncbi.nlm.nih.gov/pubmed/25800740 http://dx.doi.org/10.1093/nar/gkv204

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