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mRNA maturation in giant viruses: variation on a theme
Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402537/ https://www.ncbi.nlm.nih.gov/pubmed/25779049 http://dx.doi.org/10.1093/nar/gkv224 |
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author | Priet, Stéphane Lartigue, Audrey Debart, Françoise Claverie, Jean-Michel Abergel, Chantal |
author_facet | Priet, Stéphane Lartigue, Audrey Debart, Françoise Claverie, Jean-Michel Abergel, Chantal |
author_sort | Priet, Stéphane |
collection | PubMed |
description | Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene cluster both encode the enzyme responsible for the hairpin cleavage and the viral polyA polymerases (vPAP). Unexpectedly, the vPAPs are homodimeric and uniquely self-processive. The vPAP backbone structures exhibit a symmetrical architecture with two subdomains sharing a nucleotidyltransferase topology, suggesting that vPAPs originate from an ancestral duplication. A Poxvirus processivity factor homologue encoded by Megavirus chilensis displays a conserved 5′-GpppA 2′O methyltransferase activity but is also able to internally methylate the mRNAs’ polyA tails. These findings elucidate how the arm wrestling between hosts and their viruses to access the translation machinery is taking place in Mimiviridae. |
format | Online Article Text |
id | pubmed-4402537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44025372015-04-29 mRNA maturation in giant viruses: variation on a theme Priet, Stéphane Lartigue, Audrey Debart, Françoise Claverie, Jean-Michel Abergel, Chantal Nucleic Acids Res RNA Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene cluster both encode the enzyme responsible for the hairpin cleavage and the viral polyA polymerases (vPAP). Unexpectedly, the vPAPs are homodimeric and uniquely self-processive. The vPAP backbone structures exhibit a symmetrical architecture with two subdomains sharing a nucleotidyltransferase topology, suggesting that vPAPs originate from an ancestral duplication. A Poxvirus processivity factor homologue encoded by Megavirus chilensis displays a conserved 5′-GpppA 2′O methyltransferase activity but is also able to internally methylate the mRNAs’ polyA tails. These findings elucidate how the arm wrestling between hosts and their viruses to access the translation machinery is taking place in Mimiviridae. Oxford University Press 2015-04-20 2015-03-16 /pmc/articles/PMC4402537/ /pubmed/25779049 http://dx.doi.org/10.1093/nar/gkv224 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Priet, Stéphane Lartigue, Audrey Debart, Françoise Claverie, Jean-Michel Abergel, Chantal mRNA maturation in giant viruses: variation on a theme |
title | mRNA maturation in giant viruses: variation on a theme |
title_full | mRNA maturation in giant viruses: variation on a theme |
title_fullStr | mRNA maturation in giant viruses: variation on a theme |
title_full_unstemmed | mRNA maturation in giant viruses: variation on a theme |
title_short | mRNA maturation in giant viruses: variation on a theme |
title_sort | mrna maturation in giant viruses: variation on a theme |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402537/ https://www.ncbi.nlm.nih.gov/pubmed/25779049 http://dx.doi.org/10.1093/nar/gkv224 |
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