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Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling

Outer membrane proteins (OMPs) can induce an immune response. Omp18 (HP1125) of H. pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels. Previous studies have suggested that IFN-γ plays an important role in H. pylori clearance. However, H. pylori has multiple mechanis...

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Autores principales: Shan, Yuqun, Lu, Xingxiao, Han, Yingnan, Li, Xinpeng, Wang, Xiao, Shao, Chunhong, Wang, Lixiang, Liu, Zhifang, Tang, Wei, Sun, Yundong, Jia, Jihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402576/
https://www.ncbi.nlm.nih.gov/pubmed/25945338
http://dx.doi.org/10.1155/2015/571280
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author Shan, Yuqun
Lu, Xingxiao
Han, Yingnan
Li, Xinpeng
Wang, Xiao
Shao, Chunhong
Wang, Lixiang
Liu, Zhifang
Tang, Wei
Sun, Yundong
Jia, Jihui
author_facet Shan, Yuqun
Lu, Xingxiao
Han, Yingnan
Li, Xinpeng
Wang, Xiao
Shao, Chunhong
Wang, Lixiang
Liu, Zhifang
Tang, Wei
Sun, Yundong
Jia, Jihui
author_sort Shan, Yuqun
collection PubMed
description Outer membrane proteins (OMPs) can induce an immune response. Omp18 (HP1125) of H. pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels. Previous studies have suggested that IFN-γ plays an important role in H. pylori clearance. However, H. pylori has multiple mechanisms to avoid host immune surveillance for persistent colonization. We generated an omp18 mutant (H. pylori 26695 and H. pylori SS1) strain to examine whether Omp18 interacts with IFN-γ and is involved in H. pylori colonization. qRT-PCR revealed that IFN-γ induced Omp18 expression. qRT-PCR and western blot analysis revealed reduced expressions of virulence factors CagA and NapA in H. pylori 26695 with IFN-γ treatment, but they were induced in the Δomp18 strain. In C57BL/6 mice infected with H. pylori SS1 and the Δomp18 strain, the Δomp18 strain conferred defective colonization and activated a stronger inflammatory response. Signal transducer phosphorylation and transcription 1 (STAT1) activator was downregulated by the wild-type strain but not the Δomp18 strain in IFN-γ-treated macrophages. Furthermore, Δomp18 strain survival rates were poor in macrophages compared to the wild-type strain. We concluded that H. pylori Omp18 has an important function influencing IFN-γ-mediated immune response to participate in persistent colonization.
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spelling pubmed-44025762015-05-05 Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling Shan, Yuqun Lu, Xingxiao Han, Yingnan Li, Xinpeng Wang, Xiao Shao, Chunhong Wang, Lixiang Liu, Zhifang Tang, Wei Sun, Yundong Jia, Jihui Biomed Res Int Research Article Outer membrane proteins (OMPs) can induce an immune response. Omp18 (HP1125) of H. pylori is a powerful antigen that can induce significant interferon-γ (IFN-γ) levels. Previous studies have suggested that IFN-γ plays an important role in H. pylori clearance. However, H. pylori has multiple mechanisms to avoid host immune surveillance for persistent colonization. We generated an omp18 mutant (H. pylori 26695 and H. pylori SS1) strain to examine whether Omp18 interacts with IFN-γ and is involved in H. pylori colonization. qRT-PCR revealed that IFN-γ induced Omp18 expression. qRT-PCR and western blot analysis revealed reduced expressions of virulence factors CagA and NapA in H. pylori 26695 with IFN-γ treatment, but they were induced in the Δomp18 strain. In C57BL/6 mice infected with H. pylori SS1 and the Δomp18 strain, the Δomp18 strain conferred defective colonization and activated a stronger inflammatory response. Signal transducer phosphorylation and transcription 1 (STAT1) activator was downregulated by the wild-type strain but not the Δomp18 strain in IFN-γ-treated macrophages. Furthermore, Δomp18 strain survival rates were poor in macrophages compared to the wild-type strain. We concluded that H. pylori Omp18 has an important function influencing IFN-γ-mediated immune response to participate in persistent colonization. Hindawi Publishing Corporation 2015 2015-04-06 /pmc/articles/PMC4402576/ /pubmed/25945338 http://dx.doi.org/10.1155/2015/571280 Text en Copyright © 2015 Yuqun Shan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shan, Yuqun
Lu, Xingxiao
Han, Yingnan
Li, Xinpeng
Wang, Xiao
Shao, Chunhong
Wang, Lixiang
Liu, Zhifang
Tang, Wei
Sun, Yundong
Jia, Jihui
Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title_full Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title_fullStr Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title_full_unstemmed Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title_short Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
title_sort helicobacter pylori outer membrane protein 18 (hp1125) is involved in persistent colonization by evading interferon-γ signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402576/
https://www.ncbi.nlm.nih.gov/pubmed/25945338
http://dx.doi.org/10.1155/2015/571280
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