Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR

We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These...

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Autores principales: Sarou-Kanian, Vincent, Joudiou, Nicolas, Louat, Fanny, Yon, Maxime, Szeremeta, Frédéric, Même, Sandra, Massiot, Dominique, Decoville, Martine, Fayon, Franck, Beloeil, Jean-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402646/
https://www.ncbi.nlm.nih.gov/pubmed/25892587
http://dx.doi.org/10.1038/srep09872
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author Sarou-Kanian, Vincent
Joudiou, Nicolas
Louat, Fanny
Yon, Maxime
Szeremeta, Frédéric
Même, Sandra
Massiot, Dominique
Decoville, Martine
Fayon, Franck
Beloeil, Jean-Claude
author_facet Sarou-Kanian, Vincent
Joudiou, Nicolas
Louat, Fanny
Yon, Maxime
Szeremeta, Frédéric
Même, Sandra
Massiot, Dominique
Decoville, Martine
Fayon, Franck
Beloeil, Jean-Claude
author_sort Sarou-Kanian, Vincent
collection PubMed
description We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in β-alanine signals in the thorax of flies showing muscle degeneration.
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spelling pubmed-44026462015-04-29 Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR Sarou-Kanian, Vincent Joudiou, Nicolas Louat, Fanny Yon, Maxime Szeremeta, Frédéric Même, Sandra Massiot, Dominique Decoville, Martine Fayon, Franck Beloeil, Jean-Claude Sci Rep Article We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in β-alanine signals in the thorax of flies showing muscle degeneration. Nature Publishing Group 2015-04-20 /pmc/articles/PMC4402646/ /pubmed/25892587 http://dx.doi.org/10.1038/srep09872 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sarou-Kanian, Vincent
Joudiou, Nicolas
Louat, Fanny
Yon, Maxime
Szeremeta, Frédéric
Même, Sandra
Massiot, Dominique
Decoville, Martine
Fayon, Franck
Beloeil, Jean-Claude
Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title_full Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title_fullStr Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title_full_unstemmed Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title_short Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR
title_sort metabolite localization in living drosophila using high resolution magic angle spinning nmr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402646/
https://www.ncbi.nlm.nih.gov/pubmed/25892587
http://dx.doi.org/10.1038/srep09872
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