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Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island

Staphylococcus aureus is a major pathogen of humans and animals. The capacity of S. aureus to adapt to different host species and tissue types is strongly influenced by the acquisition of mobile genetic elements encoding determinants involved in niche adaptation. The genomic islands νSaα and νSaβ ar...

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Autores principales: Moon, Bo Youn, Park, Joo Youn, Hwang, Sun Yung, Robinson, D. Ashley, Thomas, Jonathan C., Fitzgerald, J. Ross, Park, Yong Ho, Seo, Keun Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402969/
https://www.ncbi.nlm.nih.gov/pubmed/25891795
http://dx.doi.org/10.1038/srep09784
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author Moon, Bo Youn
Park, Joo Youn
Hwang, Sun Yung
Robinson, D. Ashley
Thomas, Jonathan C.
Fitzgerald, J. Ross
Park, Yong Ho
Seo, Keun Seok
author_facet Moon, Bo Youn
Park, Joo Youn
Hwang, Sun Yung
Robinson, D. Ashley
Thomas, Jonathan C.
Fitzgerald, J. Ross
Park, Yong Ho
Seo, Keun Seok
author_sort Moon, Bo Youn
collection PubMed
description Staphylococcus aureus is a major pathogen of humans and animals. The capacity of S. aureus to adapt to different host species and tissue types is strongly influenced by the acquisition of mobile genetic elements encoding determinants involved in niche adaptation. The genomic islands νSaα and νSaβ are found in almost all S. aureus strains and are characterized by extensive variation in virulence gene content. However the basis for the diversity and the mechanism underlying mobilization of the genomic islands between strains are unexplained. Here, we demonstrated that the genomic island, νSaβ, encoding an array of virulence factors including staphylococcal superantigens, proteases, and leukotoxins, in addition to bacteriocins, was transferrable in vitro to human and animal strains of multiple S. aureus clones via a resident prophage. The transfer of the νSaβ appears to have been accomplished by multiple conversions of transducing phage particles carrying overlapping segments of the νSaβ. Our findings solve a long-standing mystery regarding the diversification and spread of the genomic island νSaβ, highlighting the central role of bacteriophages in the pathogenic evolution of S. aureus.
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spelling pubmed-44029692015-04-29 Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island Moon, Bo Youn Park, Joo Youn Hwang, Sun Yung Robinson, D. Ashley Thomas, Jonathan C. Fitzgerald, J. Ross Park, Yong Ho Seo, Keun Seok Sci Rep Article Staphylococcus aureus is a major pathogen of humans and animals. The capacity of S. aureus to adapt to different host species and tissue types is strongly influenced by the acquisition of mobile genetic elements encoding determinants involved in niche adaptation. The genomic islands νSaα and νSaβ are found in almost all S. aureus strains and are characterized by extensive variation in virulence gene content. However the basis for the diversity and the mechanism underlying mobilization of the genomic islands between strains are unexplained. Here, we demonstrated that the genomic island, νSaβ, encoding an array of virulence factors including staphylococcal superantigens, proteases, and leukotoxins, in addition to bacteriocins, was transferrable in vitro to human and animal strains of multiple S. aureus clones via a resident prophage. The transfer of the νSaβ appears to have been accomplished by multiple conversions of transducing phage particles carrying overlapping segments of the νSaβ. Our findings solve a long-standing mystery regarding the diversification and spread of the genomic island νSaβ, highlighting the central role of bacteriophages in the pathogenic evolution of S. aureus. Nature Publishing Group 2015-04-20 /pmc/articles/PMC4402969/ /pubmed/25891795 http://dx.doi.org/10.1038/srep09784 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Moon, Bo Youn
Park, Joo Youn
Hwang, Sun Yung
Robinson, D. Ashley
Thomas, Jonathan C.
Fitzgerald, J. Ross
Park, Yong Ho
Seo, Keun Seok
Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title_full Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title_fullStr Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title_full_unstemmed Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title_short Phage-mediated horizontal transfer of a Staphylococcus aureus virulence-associated genomic island
title_sort phage-mediated horizontal transfer of a staphylococcus aureus virulence-associated genomic island
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4402969/
https://www.ncbi.nlm.nih.gov/pubmed/25891795
http://dx.doi.org/10.1038/srep09784
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