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Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles

In the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irra...

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Autores principales: Niknejad, Hassan, Mahmoudzadeh, Raziyeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403054/
https://www.ncbi.nlm.nih.gov/pubmed/25901145
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author Niknejad, Hassan
Mahmoudzadeh, Raziyeh
author_facet Niknejad, Hassan
Mahmoudzadeh, Raziyeh
author_sort Niknejad, Hassan
collection PubMed
description In the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irradiation, adding of glucose and combination of both methods. The nanoparticles were prepared by desolvation procedure. Final particle size, zeta potential, FTIR, scanning electron micrograph, cellular uptake and cell toxicity of nanoparticles crosslinked with UV and/or glucose were compared with commonly crosslinked nanoparticles with GA. Moreover, drug release and stability parameters of docetaxel-loaded albumin nanoparticles were investigated. Size of all nanoparticles prepared by different methods was in the same range (100-200 nm). Zeta potential showed the same results except for those treated with UV. The results of FTIR assay were the same for all groups. Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA. Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results. The release of docetaxel from UV + glucose and GA crosslinked nanoparticles showed the same biphasic release. These data support the idea that crosslinking with a combination of UV and glucose can be a promising alternative method for production of docetaxel-loaded albumin nanoparticles with the advantage of omitting toxic GA.
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spelling pubmed-44030542015-04-21 Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles Niknejad, Hassan Mahmoudzadeh, Raziyeh Iran J Pharm Res Original Article In the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (GA) is added to stabilize the newly formed nanoparticles. Due to undesirable effects of GA, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (UV) irradiation, adding of glucose and combination of both methods. The nanoparticles were prepared by desolvation procedure. Final particle size, zeta potential, FTIR, scanning electron micrograph, cellular uptake and cell toxicity of nanoparticles crosslinked with UV and/or glucose were compared with commonly crosslinked nanoparticles with GA. Moreover, drug release and stability parameters of docetaxel-loaded albumin nanoparticles were investigated. Size of all nanoparticles prepared by different methods was in the same range (100-200 nm). Zeta potential showed the same results except for those treated with UV. The results of FTIR assay were the same for all groups. Although crosslinking by UV or glucose alone resulted in cytotoxic effects, combination of UV and glucose had less cytotoxic effects compared to GA. Cellular uptake of nanoparticles crosslinked with UV + glucose and GA showed similar results. The release of docetaxel from UV + glucose and GA crosslinked nanoparticles showed the same biphasic release. These data support the idea that crosslinking with a combination of UV and glucose can be a promising alternative method for production of docetaxel-loaded albumin nanoparticles with the advantage of omitting toxic GA. Shaheed Beheshti University of Medical Sciences 2015 /pmc/articles/PMC4403054/ /pubmed/25901145 Text en © 2015 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Niknejad, Hassan
Mahmoudzadeh, Raziyeh
Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title_full Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title_fullStr Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title_full_unstemmed Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title_short Comparison of Different Crosslinking Methods for Preparation of Docetaxel-loaded Albumin Nanoparticles
title_sort comparison of different crosslinking methods for preparation of docetaxel-loaded albumin nanoparticles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403054/
https://www.ncbi.nlm.nih.gov/pubmed/25901145
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