A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides

A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9–3.3) and 30% B (100% a...

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Detalles Bibliográficos
Autores principales: Wang, Wei, Wang, Nan, Zhang, Yu, Cai, Zheng, Chen, Qihe, He, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403573/
https://www.ncbi.nlm.nih.gov/pubmed/25969772
http://dx.doi.org/10.5402/2013/453910
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author Wang, Wei
Wang, Nan
Zhang, Yu
Cai, Zheng
Chen, Qihe
He, Guoqing
author_facet Wang, Wei
Wang, Nan
Zhang, Yu
Cai, Zheng
Chen, Qihe
He, Guoqing
author_sort Wang, Wei
collection PubMed
description A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9–3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting enzyme inhibitory peptides assay was convenient for the Iso-gradient program. The accuracy of the RT-HPLC method was verified by analyzing ACE inhibitory activity of the hydrolysate peptides of silkworm pupae protein, and the results showed that the RT-HPLC method was available for exploring new source of angiotensin I-converting enzyme inhibitory peptides rapidly and veraciously.
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spelling pubmed-44035732015-05-12 A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides Wang, Wei Wang, Nan Zhang, Yu Cai, Zheng Chen, Qihe He, Guoqing ISRN Biotechnol Research Article A convenient and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for angiotensin I-converting enzyme inhibitory peptides assay was described in this paper. The mobile phase consisted of 70% A (0.05% TFA and 0.05% triethylamine in water, pH = 2.9–3.3) and 30% B (100% acetonitrile) using an Isogradient program. The flow rate was 0.5 mL/min. The absorb wavelength was 226.5 nm; the column temperature was controlled at 25°C. This method for angiotensin I-converting enzyme inhibitory peptides assay was convenient for the Iso-gradient program. The accuracy of the RT-HPLC method was verified by analyzing ACE inhibitory activity of the hydrolysate peptides of silkworm pupae protein, and the results showed that the RT-HPLC method was available for exploring new source of angiotensin I-converting enzyme inhibitory peptides rapidly and veraciously. Hindawi Publishing Corporation 2012-10-21 /pmc/articles/PMC4403573/ /pubmed/25969772 http://dx.doi.org/10.5402/2013/453910 Text en Copyright © 2013 Wei Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Wei
Wang, Nan
Zhang, Yu
Cai, Zheng
Chen, Qihe
He, Guoqing
A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title_full A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title_fullStr A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title_full_unstemmed A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title_short A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides
title_sort convenient rp-hplc method for assay bioactivities of angiotensin i-converting enzyme inhibitory peptides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403573/
https://www.ncbi.nlm.nih.gov/pubmed/25969772
http://dx.doi.org/10.5402/2013/453910
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