Development and application of SYBR Green I real-time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B

BACKGROUND: Subgroup A, B, and J ALVs are the most prevalent avian leukosis virus (ALV). Our study attempted to develop two SYBR Green I-based real-time PCR (RT-PCR) assays for specific detection of ALV subgroup J (ALV-J) and multiplex detection of ALV subgroups A and B (ALV-A/B), respectively. RESU...

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Detalles Bibliográficos
Autores principales: Dai, Manman, Feng, Min, Liu, Di, Cao, Weisheng, Liao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403717/
https://www.ncbi.nlm.nih.gov/pubmed/25889925
http://dx.doi.org/10.1186/s12985-015-0291-7

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