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Characterization of interaction of magnetic nanoparticles with breast cancer cells
BACKGROUND: Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the int...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403785/ https://www.ncbi.nlm.nih.gov/pubmed/25880445 http://dx.doi.org/10.1186/s12951-015-0073-9 |
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author | Calero, Macarena Chiappi, Michele Lazaro-Carrillo, Ana Rodríguez, María José Chichón, Francisco Javier Crosbie-Staunton, Kieran Prina-Mello, Adriele Volkov, Yuri Villanueva, Angeles Carrascosa, José L |
author_facet | Calero, Macarena Chiappi, Michele Lazaro-Carrillo, Ana Rodríguez, María José Chichón, Francisco Javier Crosbie-Staunton, Kieran Prina-Mello, Adriele Volkov, Yuri Villanueva, Angeles Carrascosa, José L |
author_sort | Calero, Macarena |
collection | PubMed |
description | BACKGROUND: Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells. RESULTS: Cells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells. CONCLUSIONS: All these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0073-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4403785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44037852015-04-21 Characterization of interaction of magnetic nanoparticles with breast cancer cells Calero, Macarena Chiappi, Michele Lazaro-Carrillo, Ana Rodríguez, María José Chichón, Francisco Javier Crosbie-Staunton, Kieran Prina-Mello, Adriele Volkov, Yuri Villanueva, Angeles Carrascosa, José L J Nanobiotechnology Research BACKGROUND: Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells. RESULTS: Cells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells. CONCLUSIONS: All these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0073-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-02-26 /pmc/articles/PMC4403785/ /pubmed/25880445 http://dx.doi.org/10.1186/s12951-015-0073-9 Text en © Calero et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Calero, Macarena Chiappi, Michele Lazaro-Carrillo, Ana Rodríguez, María José Chichón, Francisco Javier Crosbie-Staunton, Kieran Prina-Mello, Adriele Volkov, Yuri Villanueva, Angeles Carrascosa, José L Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title | Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title_full | Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title_fullStr | Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title_full_unstemmed | Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title_short | Characterization of interaction of magnetic nanoparticles with breast cancer cells |
title_sort | characterization of interaction of magnetic nanoparticles with breast cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403785/ https://www.ncbi.nlm.nih.gov/pubmed/25880445 http://dx.doi.org/10.1186/s12951-015-0073-9 |
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