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Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum

Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741...

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Autores principales: Naqvi, Ahmad Abu Turab, Shahbaaz, Mohd, Ahmad, Faizan, Hassan, Md. Imtaiyaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403809/
https://www.ncbi.nlm.nih.gov/pubmed/25894582
http://dx.doi.org/10.1371/journal.pone.0124177
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author Naqvi, Ahmad Abu Turab
Shahbaaz, Mohd
Ahmad, Faizan
Hassan, Md. Imtaiyaz
author_facet Naqvi, Ahmad Abu Turab
Shahbaaz, Mohd
Ahmad, Faizan
Hassan, Md. Imtaiyaz
author_sort Naqvi, Ahmad Abu Turab
collection PubMed
description Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741.1) encodes 1,027 proteins, of which 444 proteins are known as hypothetical proteins (HPs), i.e., proteins of unknown functions. Here, we performed functional annotation of HPs of T. pallidum ssp. pallidum using various database, domain architecture predictors, protein function annotators and clustering tools. We have analyzed the sequences of 444 HPs of T. pallidum ssp. pallidum and subsequently predicted the function of 207 HPs with a high level of confidence. However, functions of 237 HPs are predicted with less accuracy. We found various enzymes, transporters, binding proteins in the annotated group of HPs that may be possible molecular targets, facilitating for the survival of pathogen. Our comprehensive analysis helps to understand the mechanism of pathogenesis to provide many novel potential therapeutic interventions.
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spelling pubmed-44038092015-05-02 Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum Naqvi, Ahmad Abu Turab Shahbaaz, Mohd Ahmad, Faizan Hassan, Md. Imtaiyaz PLoS One Research Article Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741.1) encodes 1,027 proteins, of which 444 proteins are known as hypothetical proteins (HPs), i.e., proteins of unknown functions. Here, we performed functional annotation of HPs of T. pallidum ssp. pallidum using various database, domain architecture predictors, protein function annotators and clustering tools. We have analyzed the sequences of 444 HPs of T. pallidum ssp. pallidum and subsequently predicted the function of 207 HPs with a high level of confidence. However, functions of 237 HPs are predicted with less accuracy. We found various enzymes, transporters, binding proteins in the annotated group of HPs that may be possible molecular targets, facilitating for the survival of pathogen. Our comprehensive analysis helps to understand the mechanism of pathogenesis to provide many novel potential therapeutic interventions. Public Library of Science 2015-04-20 /pmc/articles/PMC4403809/ /pubmed/25894582 http://dx.doi.org/10.1371/journal.pone.0124177 Text en © 2015 Naqvi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Naqvi, Ahmad Abu Turab
Shahbaaz, Mohd
Ahmad, Faizan
Hassan, Md. Imtaiyaz
Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title_full Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title_fullStr Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title_full_unstemmed Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title_short Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
title_sort identification of functional candidates amongst hypothetical proteins of treponema pallidum ssp. pallidum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403809/
https://www.ncbi.nlm.nih.gov/pubmed/25894582
http://dx.doi.org/10.1371/journal.pone.0124177
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