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Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403809/ https://www.ncbi.nlm.nih.gov/pubmed/25894582 http://dx.doi.org/10.1371/journal.pone.0124177 |
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author | Naqvi, Ahmad Abu Turab Shahbaaz, Mohd Ahmad, Faizan Hassan, Md. Imtaiyaz |
author_facet | Naqvi, Ahmad Abu Turab Shahbaaz, Mohd Ahmad, Faizan Hassan, Md. Imtaiyaz |
author_sort | Naqvi, Ahmad Abu Turab |
collection | PubMed |
description | Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741.1) encodes 1,027 proteins, of which 444 proteins are known as hypothetical proteins (HPs), i.e., proteins of unknown functions. Here, we performed functional annotation of HPs of T. pallidum ssp. pallidum using various database, domain architecture predictors, protein function annotators and clustering tools. We have analyzed the sequences of 444 HPs of T. pallidum ssp. pallidum and subsequently predicted the function of 207 HPs with a high level of confidence. However, functions of 237 HPs are predicted with less accuracy. We found various enzymes, transporters, binding proteins in the annotated group of HPs that may be possible molecular targets, facilitating for the survival of pathogen. Our comprehensive analysis helps to understand the mechanism of pathogenesis to provide many novel potential therapeutic interventions. |
format | Online Article Text |
id | pubmed-4403809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44038092015-05-02 Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum Naqvi, Ahmad Abu Turab Shahbaaz, Mohd Ahmad, Faizan Hassan, Md. Imtaiyaz PLoS One Research Article Syphilis is a globally occurring venereal disease, and its infection is propagated through sexual contact. The causative agent of syphilis, Treponema pallidum ssp. pallidum, a Gram-negative sphirochaete, is an obligate human parasite. Genome of T. pallidum ssp. pallidum SS14 strain (RefSeq NC_010741.1) encodes 1,027 proteins, of which 444 proteins are known as hypothetical proteins (HPs), i.e., proteins of unknown functions. Here, we performed functional annotation of HPs of T. pallidum ssp. pallidum using various database, domain architecture predictors, protein function annotators and clustering tools. We have analyzed the sequences of 444 HPs of T. pallidum ssp. pallidum and subsequently predicted the function of 207 HPs with a high level of confidence. However, functions of 237 HPs are predicted with less accuracy. We found various enzymes, transporters, binding proteins in the annotated group of HPs that may be possible molecular targets, facilitating for the survival of pathogen. Our comprehensive analysis helps to understand the mechanism of pathogenesis to provide many novel potential therapeutic interventions. Public Library of Science 2015-04-20 /pmc/articles/PMC4403809/ /pubmed/25894582 http://dx.doi.org/10.1371/journal.pone.0124177 Text en © 2015 Naqvi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Naqvi, Ahmad Abu Turab Shahbaaz, Mohd Ahmad, Faizan Hassan, Md. Imtaiyaz Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum |
title | Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
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title_full | Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
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title_fullStr | Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
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title_full_unstemmed | Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
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title_short | Identification of Functional Candidates amongst Hypothetical Proteins of Treponema pallidum ssp. pallidum
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title_sort | identification of functional candidates amongst hypothetical proteins of treponema pallidum ssp. pallidum |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403809/ https://www.ncbi.nlm.nih.gov/pubmed/25894582 http://dx.doi.org/10.1371/journal.pone.0124177 |
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